商品编号


0500-201



品牌


Reachbio



公司


Reachbio



公司分类


Human Peripheral Blood Cells




10 million, 100 million, 25 million, 50 million


pa_unit-size

25-million

商品信息

Product Description

Human Peripheral Blood Mononuclear Cells (PBMCs) – Light Density





Catalog

Number



Selection

Type



Unit Size

(cells per vial)


Product

Info Sheet



0500-101

none

10 million

download



0500-201

none

25 million

download



0500-301

none

50 million

download



0500-401

none

100 million

download




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Cell Enrichment

Ficoll Density Centrifugation

Human light density peripheral blood mononuclear cells (PBMCs) are obtained by density centrifugation (Ficoll) of a leukapheresis product.

Sample Collection and Processing

Peripheral blood cells are collected from normal human volunteers via leukapheresis (COBE
?
Spectra MNC collection protocol or equivalent).The mononuclear cell-rich leukocyte layer (leukapheresis product) is continually aspirated by a low flow rate collection pump directly into a collection bag. MNCs are then further enriched by density centrifugation.


Peripheral blood cells are processed within 24-30 hours of collection.
Donors have been screened and found to be negative for a standard panel of infectious agents that at minimum includes HIV, HBV, and HCV. Depending on the specific lot, other infectious agents may also have been tested for (Certificate of Analysis includes lot-specific testing information).
?

St
ABI
lity and Storage
Peripheral blood mononuclear cells (PBMCs) are cryopreserved in CryoStor? CS5, a serum-free freezing medium. Cells are stable for long-term storage at -152
o
C or in the gaseous phase of liquid nitrogen. Storage at -80
o
C is not recommended. Once thawed, the cells must be used immediately and have a finite life span in culture.
?

Intended Use
FOR IN VITRO RESEARCH USE ONLY. NOT INTENDED FOR HUMAN OR VETERINARY USE IN VIVO. NOT TO BE USED FOR ANY DIAGNOSTIC, THERAPEUTIC OR CLINICAL APPLICATIONS WHATSOEVER. ?
Additional Information



Weight

1 lbs



Species

Human




Tissue Source

Peripheral Blood




Cell Type

Mononuclear Cells (MNCs)




Selection Type

Ficoll




Identifying Surface
Marker

n/a




Unit Size (million cells/vial)

10 million, 100 million, 25 million, 50 million