商品编号


72023



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Immunotherapy



Size

96 reactions

商品信息













Background:
L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. Tryptophan 2,3 dioxygenase (TDO) is upregulated in many tumors, providing cancer cells with an avenue for immune evasion.




Description:
The
TDO Inhibitor Screening Assay Kit
is designed to measure
TDO
enzyme inhibition. The kit comes in a convenient format, with enough reaction solution and enzyme to perform a total of 100 reactions. The TDO Inhibitor Screening Assay Kit is simple to use. Inhibitor and enzyme are added to a sample containing L-Trp substrate. After a room temperature incubation activity is measured by measuring the absorption of reaction product at &lam
BD
a;?= 320-325 nm.




Synonym(s):
TDO2, tryptophan 2,3 dioxygenase




Format:

COMPONENTS:





Instructions for use:
See product datasheet for detailed protocol.




Storage / St
ABI
lity:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.




Application(s):
Useful for the study of TDO enzymology, screening inhibitors and selectivity profiling.




Reference(s):
Li, J.S., et al. Arch Insect Biochem Physiol. 2007 Feb; 64(2): 74–87




Notes:

MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:

Spectrophotometer capable of measuring absorbance at = 320-325 nm.




Warning(s):

DMSO >0.5%, strong acids or bases, ionic detergents, high salt
Avoid freeze/thaw cycles.?




Scientific Category:
Immunotherapy, Metabolic enzyme




Product Type:
Assay Kit




Data shown is lot-specific.
Contact us
for specific information on other lots.