商品编号


72026



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Immunotherapy



Size

96 reactions


商品信息













Background:
There have been a number of studies defining a role for PD-L1 in the regulation of immune tolerance and cancer immunity through binding to T cell-expressed PD-1. Interestingly, PD-L1 has also been shown to interact with B7-1, also known as CD80. Like PD- 1:PD-L1 interaction, B7-1:PD-L1 signaling also leads to inhibition of T cell activation, proliferation, and cytokine production, suggesting that this pathway may also serve as a target in cancer immunotherapy and inflammatory diseases.




Description:
The PD-L1:B7-1[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of the PD-L1:B7-1 interaction. The key to this kit is the high sensitivity of detection of biotin-labeled B7-1 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, PD-L1 is coated on a 96-well plate. Next, B7-1-biotin is incubated with PD-L1 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.




Synonym(s):
Programmed Cell Death 1 Ligand 1, CD274, B7 homolog 1 (B7-H1), CD80, CTLA-4 Counter-Receptor B7.1




Supplied As:
This kit comes in a convenient 96-well format, with biotin-labeled B7-1 (CD80), purified PD-L1, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.




Format:






Instructions for use:
See assay kit data sheet for detailed protocol




Storage / St
ABI
lity:
Stable for one year from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.




Application(s):
This kit is useful for screening for inhibitors of PD-L1 binding to B7-1.




Reference(s):
1. Butte, M.J.,
et al., Immunity
. 2007;
27(1)
: 111-122.
2. Butte, M.J.
et al., Mol. Immunol
. 2008;
45
: 3567-3572.




Warning(s):
Avoid freeze/thaw cycles




Scientific Category:
Immunotherapy




Product Type:
Assay Kit




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for specific information on other lots.