商品编号


72041



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Immunotherapy



Size

96 reactions

商品信息













Background:
L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. Indoleamine 2,3- dioxygenases (IDO1 & IDO2), two of the rate limiting enzymes in this pathway, are upregulated in many tumors, providing cancer cells with an avenue for immune evasion.




Description:
The
Mouse IDO1 Inhibitor Screening Assay Kit
is designed to measure inhibition of the murine IDO1 enzyme. This kit is simple to use. Inhibitor and enzyme are added to a sample containing L-Trp substrate. After a room temperature incubation, activity is determined by measuring the absorption of reaction product at l=320-325 nm.




Synonym(s):
Indoleamine 2,3-dioxygenase 1, IDO, IDO1




Supplied As:
The kit comes in a convenient format, with enough reaction solution and enzyme to perform a total of 100 reactions.




Contraindications:
DMSO >0.5%, strong acids or bases, ionic detergents, high salt




Format:

COMPONENTS:





Instructions for use:
See assay kit data sheet for detailed protocol




Storage / St
ABI
lity:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.




Application(s):
Useful for the study of mouse IDO1 enzymology, screening inhibitors and selectivity profiling.




Reference(s):

1. Koblish, H.K.,
et al., Mol Cancer Ther,
2010;
9(2)
: 489-98
2. Liu, X.,
et al., Blood.
2010;
115(17)
: 3520-3530.
3. Seegers, N.,
et al. J Biomol Screen.
2014;
19(9)
:1266-74.




Warning(s):
Avoid freeze/thaw cycles.




Scientific Category:
Immunotherapy




Product Type:
Assay Kit




Data shown is lot-specific.
Contact us
for specific information on other lots.