商品编号


52069



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Methyltransferase(s)



Size

384 reactions

商品信息













Description:
The PRMT9 Homogeneous Assay Kit is designed to measure PRMT9 activity for screening and profiling applications. PRMT9 is a histone methyltransferase that exhibits methylation activity toward an arginine residue of SAP145. The PRMT9 Homogeneous Assay Kit comes in a convenient AlphaLISA? format, with GST-tagged PRMT9 substrate, primary antibody, methylation assay buffer, and purified PRMT9 for 384 enzyme reactions.

The key to the PRMT9 Homogeneous Assay Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing PRMT9 enzyme is incubated with the substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by re
ADI
ng the Alpha-counts.




Supplied As:
Kit comes in a convenient AlphaLISA? format, with primary antibody, PRMT9 assay buffer, and purified PRMT9 for 384 enzyme reactions.




Contraindications:
Green and blue dyes that absorb light in the AlphaScreen? signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen? assays.




Format:

COMPONENTS:





Instructions for use:
See assay kit data sheet for detailed protocol.




Storage / St
ABI
lity:

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.




Application(s):
Great for studying enzyme kinetics and HTS applications.




Reference(s):
Li Y., et al., J. Neurosci. 2015; 35(37): 12890-12902.




Warning(s):
Avoid freeze/thaw cycles.




Scientific Category:
Methyltransferase




Product Type:
Assay Kit




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