商品编号


52171



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Methyltransferase(s)



Size

384 reactions

商品信息













Background:
PRMT5 is a histone methyltransferase that exhibits methylation activity toward H4-R3. PRMT5 is a critical regulatory-protein linked to genome organization, cell cycle regulation, and stem cell differentiation. PRMT5 transfers methyl groups from S-adenosylmethoinine (SAM) to form mono and symmetric di-methylated arginine residues in histone proteins, transcription factors, and other regulatory proteins. Given its regulatory function, PRMT5 is believed to contribute to tum
Origen
esis. Its dysregulated activity has been associated with many cancers including lymphomas, lung, and breast cancer. Additionally, PRMT5 has been linked to other diseases including kidney and heart disease as well as neurological disorders such as Huntington’s and Alzheimer’s diseases.




Description:
The PRMT5 TR-FRET Assay Kit is designed to measure PRMT5 activity in a homogeneous 384 reaction format. PRMT5 is a histone methyltransferase that exhibits methylation activity toward H4-R3. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The key to the PRMT5 TR-FRET Assay Kit is a highly specific antibody that recognizes methylated substrate.




Synonym(s):
PRMT5, HRMT1L5, IBP72, JBP1, SKB1




Supplied As:
The PRMT5 TR-FRET Assay Kit comes in a convenient format, with histone H4 peptide substrate, a Eu-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, FRET detection buffer, dye-labeled acceptor, and purified PRMT5.




Format:

COMPONENTS:






Instructions for use:
See assay kit data sheet for detailed protocol.




Storage / St
ABI
lity:

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.




Application(s):
Great for studying enzyme kinetics and HTS applications.




Reference(s):
Yang, Y., Bedford, M.T. 2013. Nat Rev Cancer. 13(1):37-50.




Warning(s):
Avoid freeze/thaw cycles.




Scientific Category:
Methyltransferase




Product Type:
Assay Kit




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