商品编号


72021



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Immunotherapy



Size

96 Reactions

商品信息













Background:
L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. Indoleamine 2,3- dioxygenases (IDO1 & IDO2), two of the rate limiting enzymes in this pathway, are upregulated in many tumors, providing cancer cells with an avenue for immune evasion.




Description:
The
IDO1 Inhibitor Screening Assay Kit
is designed to measure
IDO1
enzyme inhibition. The
IDO1 Assay Kit
is simple to use. Inhibitor and enzyme are added to a sample containing L-Trp substrate. After a room temperature incubation, activity is determined by measuring the absorption of reaction product at &lam
BD
a;=321 nm.




Synonym(s):
Indoleamine 2,3-dioxygenase 1




Supplied As:
The kit comes in a convenient format, with enough reaction solution and enzyme to perform a total of 100 reactions.




Contraindications:
DMSO >0.5%, strong acids or bases, ionic detergents, high salt




Format:

COMPONENTS:





Instructions for use:
See assay kit data sheet for detailed protocol




Storage / St
ABI
lity:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.




Application(s):
Useful for the study of IDO1 enzymology, screening inhibitors, and selectivity profiling.




Reference(s):

1. Liu, X.,
et al., Blood.
2010;
115(17):
3520-3530.
2. Seegers, N.,
et al. J Biomol Screen
. 2014;
19(9):
1266-74.

Application Reference(s):
1.?
Synthesis and Molecular Modeling Studies of N'-Hydroxyindazolecarboximidamides as Novel Indoleamine 2,3-Dioxygenase 1 (IDO1) Inhibitors
(2017)




Warning(s):
Avoid freeze/thaw cycles.




Scientific Category:
Immunotherapy




Product Type:
Assay Kit




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Contact us
for specific information on other lots.