商品编号


72056



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


New Products



Size

384 reactions

商品信息













Background:
Tryptophan 5-hydroxylase 1 and 2 (TPH1 and TPH2) are enzymes that catalyze the monooxygenation of tryptophan to 5-hydroxytryptophan (5-HTP) which is subsequently converted to
SERO
tonin. By catalyzing the rate-limiting step in the biosynthesis of
SERO
tonin, these enzymes also play key roles in the signaling of other neurotransmitters downstream of
SERO
tonin synthesis, like melatonin. Additionally, TPH enzymes may play a role in immune regulation through tryptophan depletion.




Description:
The TPH1 Assay Kit is designed to measure TPH1 enzyme inhibition in a 384 reaction format. This fluorescence-based assay kit is especially suitable for high throughput screening applications. The procedure is quick, straightforward, and simple - just mix two solutions, incubate, add quenching solution, and measure the fluorescence.




Synonym(s):
tryptophan 5-hyrdoxylase, TRPH




Format:

COMPONENTS:






Instructions for use:
See product datasheet for detailed protocol.




Storage / St
ABI
lity:
At least 6 months from date of receipt when stored as directed.




Application(s):
Great for screening small molecular inhibitors for drug discovery and HTS applications.




Reference(s):

1. Moran G. R., et al. J. Biol. Chem. 1998; 273:12259-12266.
2. Nowak E. C., et al., J. Exp. Med. 2012; 209:2127-2135.




Notes:

MATERIALS REQUIRED BUT NOT SUPPLIED:

Microplate reader capable of measuring Fluorescence




Warning(s):
Avoid freeze/thaw cycles.




Scientific Category:
Metabolic Enzyme, Immunotherapy




Product Type:
Assay Kit




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