商品编号


52063



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


New Products



Size

384 reactions

商品信息













Description:
The SMYD3 Homogeneous Assay Kit is designed to measure SMYD3 activity for screening and profiling applications. SMYD3 is a histone methyltransferase that promotes diand trimethylation of lysine4 of histone H3. It also exhibits methylation activity toward a lysine residue of MAPK/ERK Kinase Kinase 2 (MAP3K2). The key to the SMYD3 Homogeneous Assay Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing SMYD3 enzyme is incubated with the substrate for three hours. Next, acceptor beads and primary antibody are added, then donor beads, followed by re
ADI
ng the Alpha-counts.




Synonym(s):
KMT3E




Supplied As:
The SMYD3 Homogeneous Assay Kit comes in a convenient AlphaLISA? format, with GST-tagged SMYD3 substrate, primary antibody, methylation assay buffer, and purified SMYD3 for 384 enzyme reactions.




Contraindications:
Green and blue dyes that absorb light in the AlphaScreen? signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen? assays.




Format:

COMPONENTS:





Instructions for use:
See assay kit data sheet for detailed protocol.




Storage / St
ABI
lity:
12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.




Application(s):
Great for studying enzyme kinetics and HTS applications.




Reference(s):
1. Dillon, S.C.,
et al.
2005.
Genome
BIOLOG
y

6
:227.
2. Silva, F.P.,
et al.
2008.
Oncogene

27
:2686.




Warning(s):
Avoid freeze/thaw cycles.




Scientific Category:
Methyltransferase




Product Type:
Assay Kit




Data shown is lot-specific.
Contact us
for specific information on other lots.