商品编号


53030



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Methyltransferase(s)



Size

96 reactions

商品信息













Background:
Abnormal methylation of H3K27 is associated with a number of cancers, including breast, colorectal, and Non-Hodgkins lymphoma. The wild-type enzymatic activity of the EZH2 complex modifies Lysine 27 with two methyl groups while mutated forms of the EZH2 complex have altered activity.




Description:
The Histone H3 Dimethyl-K27 Detection Kit is designed to detect dimethylation of lysine 27 of Histone H3 (H3K27me2) in cell lysates. H3K27 is the substrate of the EZH2 methyltransferase complex. The kit comes in a convenient format, with a 96-well plate for coating with cell lysate, a detection antibody against the dimethylated K27 residue of Histone H3, and HRP labeled substrates.




Format:

COMPONENTS:






Instructions for use:
See product datasheet for detailed protocol.




Storage / St
ABI
lity:
One year from date of receipt when stored as directed.




Application(s):
Detection of H3K27(Me2) in cell lysates




Notes:

MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
Luminometer or fluorescent microplate reader capable of re
ADI
ng chemiluminescence
Rotating or rocker platform
RIPA lysis buffer (
Pierce
, #89900) with protease inhibitor (PI) cocktail (1x) and 1 mM Phenylmethanesulfonyl fluoride (PMSF)
BCA Protein Assay Kit (
Pierce
, #23227)




Scientific Category:
Methyltransferase




Product Type:
Assay Kit




Data shown is lot-specific.
Contact us
for specific information on other lots.