商品编号


50022



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Deacetylase(s)



Size

96 reactions

商品信息













Background:
Sirtuins are NAD+ dependent class III histone deacetylases that regulate important
BIOLOG
ical processes including metabolism and aging. In human, there are seven isoforms of Sirtuins, SIRT1 to SIRT7. Four of the isoforms, SIRT4 to SIRT7, have no detectable or very weak deacetylase activity. Sirtuin 6 (SIRT6) is shown to be a potent defatty-acylase of lysine residues.




Description:
The Sirtuin 6 Fluorogenic Assay Kit is a complete assay system designed to measure SIRT6 activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent SIRT6 activity measurements. In addition, the kit includes purified SIRT6 enzyme and a SIRT inhibitor, Nicotinamide, for use as a positive and negative control, respectively. The Fluorogenic SIRT6 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the r
ADI
oactivity, extraction, and chromatography aspects of tr
ADI
tional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the SIRT6 activity level. First, the Sirtuin 6 fluorometric substrate, containing a myristoylated lysine side chain, is incubated with purified SIRT6 enzyme. The demyristoylation sensitizes the substrate so subsequent treatment with the SIRT Developer produces a fluorophore that can then be measured using a fluorescence reader.




UniProt
Q8N6T7




Synonym(s):
SIRT6, Sirtuin 6, SIRT, SIRT 6, Fluorogenic Sirtuin 6 Assay Kit




Supplied As:
Kit comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent SIRT6 activity measurements. In addition, the kit includes purified SIRT6 enzyme and a SIRT inhibitor, Nicotinamide, for use as a positive and negative control,




Format:





Instructions for use:
See assay kit data sheet for detailed protocol




Storage / St
ABI
lity:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.




Application(s):
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.




Reference(s):
1. Hu, J. et al., Org Miomol Chem, 2013. 11(32):5213-5216.




Warning(s):
Avoid freeze/thaw cycles.




Scientific Category:
Deacetylase




Product Type:
Assay Kit




Data shown is lot-specific.
Contact us
for specific information on other lots.