商品编号


72014



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Cell Surface Receptor(s)



Size

384 reactions

商品信息













Description:
The PD-1:PD-L1 Homogeneous Assay Kit is designed to measure the inhibition of PD-1 binding to PD-L1. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing PD-1 and an inhibitor of choice is incubated with the biotinylated PD-L1 for 60 minutes. Next, acceptor beads are added, then donor beads, followed by re
ADI
ng the Alpha-counts.




Synonym(s):
Programmed cell death 1, PDCD1, PD-1, PD1, SLEB2, CD279, HPD-L, Programmed Cell Death 1 Ligand 1,CD274 and B7 homolog 1 (B7-H1), PDCD1 ligand 1, programmed death ligand 1, PDCD1L1




Supplied As:
The PD-1:PD-L1 Homogeneous Assay Kit comes in a convenient AlphaLISA? format with purified biotinylated PD-L1, FLAG-tagged PD-1, and assay buffer to perform a total of 384 reactions.




Contraindications:
Only limited amounts of DMSO can be included, as it has been shown to disrupt PD-1-PD-L1 interaction. Avoid green and blue dyes that absorb light in the AlphaScreen signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen assays.




Format:

COMPONENTS:





Instructions for use:
See product datasheet for detailed assay protocol.




Storage / St
ABI
lity:
At least one year from date of receipt when stored as directed.




Application(s):
Useful for screening for inhibitors of PD-1 binding to PD-L1




Reference(s):
1. Lin, D., et al. Proc Natl Acad Sci U.S.A. 2008, 105: 3011-3016.
2. Keir, M.E. et al. Annu. Rev. Immunol. 2008, 26: 677-704.




Notes:

MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:

AlphaLISA FLAG acceptor beads, 5 mg/ml (
Perkinelmer
#AL112C)
AlphaScreen Streptavidin-conjugated donor beads, 5 mg/ml (
Perkinelmer
#6760002S)
Optiplate -384 (
Perkinelmer
#6007290)
AlphaScreen microplate reader
Adjustable micropipettor and sterile tips




Warning(s):
Avoid freeze/thaw cycles.




Scientific Category:
Immunotherapy




Product Type:
Assay Kit




Data shown is lot-specific.
Contact us
for specific information on other lots.