商品编号


72028



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


New Products



Size

384 reactions

商品信息













Description:
The PD-1[Biotinylated]:PD-L1 Homogeneous Assay Kit is designed to measure the inhibition of PD-1 binding to PD-L1. The PD-1[Biotinylated]:PD-L1 Homogeneous Assay Kit comes in a convenient AlphaLISA? format with purified biotinylated PD-1, FLAG-tagged PD-L1, and assay buffer to perform a total of 384 reactions. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing biotinylated PD-1 and an inhibitor of choice is incubated with the FLAG-tagged PD-L1 for 60 minutes. Next, acceptor beads are added, then donor beads, followed by re
ADI
ng the Alpha-counts.




Synonym(s):
Programmed cell death 1, PDCD1, PD-1, PD1, SLEB2, CD279, HPD-L and Programmed Cell Death 2 Ligand 2, PDL2, PD-L2, CD273, PDCDL1G2, B7-DC




Contraindications:
Only limited amounts of DMSO can be included, as it has been shown to disrupt PD-1-PD-L1 interaction. Avoid green and blue dyes that absorb light in the AlphaScreen signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen assays.




Format:

COMPONENTS:






Instructions for use:
See assay kit data sheet for detailed protocol




Storage / St
ABI
lity:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.




Application(s):
Useful for screening for inhibitors of PD-1 binding to PD-L1




Reference(s):

1. Lin, D.,
et al. Proc Natl Acad Sci U.S.A.
2008,
105
: 3011-3016.
2. Keir, M.E.
et al. Annu. Rev. Immunol.
2008,
26
: 677-704.




Warning(s):
Avoid freeze/thaw cycles




Scientific Category:
Immunotherapy




Product Type:
Assay Kit




Data shown is lot-specific.
Contact us
for specific information on other lots.