商品编号


72042



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


New Products



Size

96 reactions

商品信息













Background:
L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. Indoleamine 2,3- dioxygenases (IDO1 & IDO2), two of the rate limiting enzymes in this pathway, are upregulated in many tumors, providing cancer cells with an avenue for immune evasion.




Description:
The
IDO2 Inhibitor Screening Assay Kit
is designed to measure mouse
IDO2
enzyme inhibition. The
Mouse IDO2 Inhibitor Screening Assay Kit
is simple to use. Inhibitor and enzyme are added to a sample containing L-Trp substrate. After a room temperature incubation, activity is determined by measuring the absorption of reaction product at l=320 – 325 nm. BACKGROUND: L-tryptophan




Synonym(s):
Indoleamine 2,3-dioxygenase 2, IDO-2, IDO




Supplied As:
The kit comes in a convenient format with enough reaction solution and enzyme to perform a total of 100 reactions.




Contraindications:
DMSO >0.5%, strong acids or bases, ionic detergents, high salt




Format:

COMPONENTS:





Instructions for use:
See assay kit data sheet for detailed protocol.




Storage / St
ABI
lity:
At least 6 months from date of receipt when stored as directed.




Application(s):
Useful for the study of mouse IDO2 enzymology, screening inhibitors, and selectivity profiling.




Reference(s):
1. Liu, X.,
et al., Blood.
2010;
115(17)
: 3520-3530.
2. Seegers, N.,
et al. J. Biomol. Screen.
2014;
19(9)
: 1266-74.




Notes:

Materials required but not supplied:
- Spectrophotometer capable of measuring absorbance at &lam
BD
a;=320 – 325 nm
- Adjustable micropipettor and sterile tips




Warning(s):
Avoid freeze/thaw cycles.




Scientific Category:
Immunotherapy




Product Type:
Assay Kit




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Contact us
for specific information on other lots.