商品编号


72050



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


New Products



Size

50 reactions

商品信息













Background:
L-Trp is an essential amino acid necessary for protein synthesis in mammalian cells, and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. Indoleamine 2,3- dioxygenases (IDO1 & IDO2), two of the rate limiting enzymes in this pathway, are upregulated in many tumors, providing cancer cells with an avenue for immune evasion.

Inhibitors of IDO1 can bind either irrevers
IBL
y (covalent binding to the protein) or revers
IBL
y (non-covalent association) to the enzyme. The IDO1 Inhibitor Mechanism of Action Assay Kit allows researchers to determine the mechanism of IDO1 inhibitor binding. At high concentrations, e.g., 10x the IC50 concentration of the inhibitor, both revers
IBL
e and irrevers
IBL
e inhibitors will interfere with IDO1 activity. However, after sufficient dilution, e.g. to 0.3x the IC50 concentration, revers
IBL
e inhibitors will dissociate, relieving inhibition of the IDO1 enzyme. Irrevers
IBL
e inhibitors will not dissociate, and will continue to inhibit the IDO1 enzyme at the same level. Therefore, by monitoring enzymatic activity after inhibitor dilution, the mechanism of binding to the IDO1 protein can be determined.




Description:
The IDO1 Inhibitor Mechanism of Action (MoA) Assay Kit is designed to determine the mechanism of IDO1 enzyme inhibition (e.g., revers
IBL
e or irrevers
IBL
e inhibition). The IDO1 Mechanism of Action Assay Kit is simple to use. Inhibitor and enzyme are preincubated allowing the inhibitor to bind. After preincubation, the mixture is diluted into reaction buffer containing the L-tryptophan (L-Trp) substrate and all necessary coupled reaction components. This allows revers
IBL
e inhibitors to dissociate, while irrevers
IBL
e inhibitors remain associated with the IDO1 enzyme. IDO1 activity is determined by measuring the absorption of the reaction product at =320 – 325 nm.




Synonym(s):
Indoleamine 2,3-dioxygenase 1, IDO




Supplied As:
The kit comes in a convenient format with enough reaction solution and enzyme to perform a total of 50 reactions.




Contraindications:
DMSO > 1%, strong acids or bases, ionic detergents, and high salt.




Format:

COMPONENTS:





Instructions for use:
See assay kit data sheet for detailed protocol




Storage / St
ABI
lity:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.




Application(s):
Useful for the study of IDO1 enzymology, screening inhibitors, and selectivity profiling.




Reference(s):

1. Liu, X.,
et al., Blood.
2010;
115(17)
: 3520-3530.
2. Seegers, N.,
et al. J Biomol Screen.
2014;
19(9)
: 1266-74.
3. Strelow, J.,
et al. Assay Guidance Manual
. 2012




Warning(s):
Avoid freeze/thaw cycles.




Scientific Category:
Immunotherapy




Product Type:
Assay Kit




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for specific information on other lots.