商品编号


60383



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Phosphodiesterase(s)



Size

384 reactions

商品信息













Background:
Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. PDE3B, also known as cGMP-inhibited phosphodiesterase, is involved in mediating the antilipolytic and anti glycogenlytic effects of insulin in
ADI
pose and liver tissues. PDE3B can hydrolyze both cAMP and cGMP.




Description:
The PDE3B Assay Kit is designed for identification of PDE3B inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE3B to the binding agent. The key to the PDE3B Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE3B reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE3B for 1 hour. Second, binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.




Synonym(s):
PDE3B, phosphodiesterase 3B




Supplied As:
The PDE1C inhibitor screening assay kit comes in a convenient 96-well format, including purified PDE1C enzyme, fluorescently labeled PDE1C substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions.




Format:

COMPONENTS:






Instructions for use:
See assay kit data sheet for detailed protocol.




Storage / St
ABI
lity:

At least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.




Application(s):
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.




Reference(s):
Chandrasekaran, A.,
et al., Cell Signal
. 2008;
20(1)
:139-53.




Notes:

MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
Fluorescent microplate reader capable to measure fluorescence polarization




Warning(s):
Avoid freeze/thaw cycles.




Scientific Category:
Phosphodiesterase




Product Type:
Assay Kit




Data shown is lot-specific.
Contact us
for specific information on other lots.