商品编号


52067



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Methyltransferase(s)



Size

96 reactions

商品信息













Description:
The EZH2 (A738T) Chemiluminescent Assay Kit is designed to measure activity of the mutant EZH2 complex (EZH2 (A738T)/EED/SUZ12/RbAp48/ AEBP) for screening and profiling purposes. The EZH2 (A738T) Chemiluminescent Assay Kit comes in a convenient format, with wells precoated with histone H3 peptide substrate, an antibody against methylated K27 residue of Histone H3, the secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and purified EZH2 (A738T) complex for 100 enzyme reactions. The key to the EZH2 (A738T) Assay Kit is a highly specific antibody that recognizes methylated Histone H3K27. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the strips are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.




Supplied As:
Kit comes in a convenient format, with wells precoated with histone H3 peptide substrate




Format:

COMPONENTS







Storage / St
ABI
lity:
One year from date of receipt when stored as directed.




Application(s):
Great for studying enzyme kinetics and HTS applications.




Reference(s):
1. Dillon, S.C., et al. Genome
BIOLOG
y 2005; 6:227.
2. Morin, R.D., et al. Nat Genet. 2010, 42(2):181.




Warning(s):
Avoid freeze/thaw cycles.




Scientific Category:
Methyltransferase




Product Type:
Assay Kit




Data shown is lot-specific.
Contact us
for specific information on other lots.