商品编号


51056



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Methyltransferase(s)



Size

96 reactions

商品信息













Description:
The SetDB1 Chemiluminescent Assay Kit is designed to measure SetDB1 activity for screening and profiling applications. The key to the SetDB1 Direct Activity Assay Kit is a highly specific antibody that recognizes the methylated K9 residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the strips are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.




UniProt
Q15047




Synonym(s):
Histone-lysine Nmethyltransferase SETDB1, SET domain bifurcated 1, KMT1E, ERG-associated protein with SET domain (ESET)




Supplied As:
Kit comes in a convenient format, with wells precoated with histone H3 peptide substrate, antibody against methylated lysine residue of Histone H3, secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase buffer, and purified enzyme




Format:





Instructions for use:
See assay kit data sheet for detailed protocol.




Storage / St
ABI
lity:
One year from date of receipt when stored as directed.




Application(s):
Great for studying enzyme kinetics and HTS applications.




Reference(s):
Dillon SC, Zhang X, Trievel RC, Cheng X. Genome
BIOLOG
y 2005; 6:227.




Notes:

MATERIALS REQUIRED BUT NOT SUPPLIED:
TBST buffer (1 x TBS, pH 8.0, containing 0.05% Tween-20)
Luminometer or fluorescent microplate reader capable of re
ADI
ng chemiluminescence
Adjustable micropipettor and sterile tips
Rotating or rocker platform




Warning(s):
Avoid freeze/thaw cycles.




Scientific Category:
Methyltransferases




Product Type:
Assay Kits




Data shown is lot-specific.
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