商品编号


79309



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Metabolic Enzymes



Size

96 reactions

商品信息













Background:
Isocitrate dehydrogenases are enzymes that catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations in isocitrate dehydrogenases 1 and 2 (IDH1 and IDH2) have been reported in a multitude of human cancers. While the wild type IDH2, produces α-ketoglutarate (α-KG) through the reduction of NADP+ to NADPH, the IDH2(R140Q) mutant catalyzes conversion of α-ketoglutarate to 2-hydroxy-glutarate (2-HG) by oxidizing NADPH to NADP+. Genomic studies have identified the IDH2(R140Q) mutation in various cancer types, including as glioma, chondrosarcoma, AML as well as small percentage of prostate, lung and colon cancers.




Description:
The IDH2(R140Q) Assay Kit is designed to measure IDH2(R140Q) activity for screening and profiling applications by measuring NADPH consumption.




Synonym(s):
Isocitrate Dehydrogenase 2, IDH2, Oxalosuccinate Decarboxylase, IDHM




Supplied As:
The IDH2(R140Q) Assay Kit comes in a convenient 96-well format, with enough purified recombinant IDH2(R140Q) enzyme, α-KG, NADPH, NADPH detection reagents, and assay buffer for 100 enzyme reactions.




Format:





Instructions for use:
See assay kit data sheet for detailed protocol.




Storage / St
ABI
lity:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.




Application(s):
Useful for screening small molecular inhibitors for drug discovery and HTS applications.




Reference(s):

Wang, F.
et. al. Science

340
:622-626 (2013).




Warning(s):
Avoid freeze/thaw cycles.




Scientific Category:
Metabolic Enzymes




Product Type:
Assay Kit




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