商品编号


40250



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Kinase(s)



Size

10 ?g

商品信息





Species: Human

Host Species: Sf9 insect cells

MW: 53.4 kDa





Genbank #: NM_000222

Tag(s): N-terminal His-tag

a.a: 544–976(end)





Description:
Human Kit (GenBank Accession No. NM_000222), a.a. 544-976(end) with N-terminal His-tag, MW=53.4 kDa, expressed in a Baculovirus infected Sf9 cell expression system.




Synonym(s):
KIT, cKIT, c-KIT, PBT, CD117, Piebald Trait, SCFR, Mast/Stem Cell Growth Factor Receptor




Specific Activity:
16 U/mg.




Unit Definition:
One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of tyrosine substrate per minute at pH 7.4 and 30°C.




Assay Conditions:
50 mM HEPES, pH 7.4, 3 mM MgCl
2
, 3 mM MnCl
2
, 1 mM DTT, 3 ?M Na orthovanadate, 0.1 mM ATP, 30 ?g/ml Poly (Glu:Tyr)4:1 substrate, and 4 ?g /ml recombinant cKit.




Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole




Format:
Aqueous buffer solution




Storage / St
ABI
lity:
>6 months at –80°C




Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.




Reference(s):
1. Picardo M Cardinali G. J Invest Dermatol.2011 Jun;131(6):1182-5.
2. Yarden, Y., et al. EMBO J. 6 (11), 3341-3351 (1987)




Warning(s):
Avoid freeze/thaw cycles




Scientific Category:
Kinase/Receptor Tyrosine




Product Type:
Recombinant Protein




Data shown is lot-specific.
Contact us
for specific information on other lots.