商品编号


40251



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Kinase(s)



Size

10 ?g

商品信息





Species: Human

Host Species: Sf9 insect cells

MW: 53.4 kDa





Genbank #: NM_000222

Tag(s): N-terminal His-tag

a.a: 544–976(end)





Description:
Human Kit (V654A) (GenBank Accession No. NM_000222), a.a. 544-976(end) with substitution of valine654 to alanine and N-terminal His-tag, MW=53.4 kDa, expressed in an Baculovirus infected Sf9 cell expression system.




UniProt
P10721




Synonym(s):
KIT, cKIT, c-KIT, PBT, CD117, Piebald Trait, SCFR, Mast/Stem Cell Growth Factor Receptor




Specific Activity:
89 pmol/min/μg




Unit Definition:
One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of tyrosine substrate per minute at pH 7.4 and 30°C.




Assay Conditions:
Enzyme reaction is conducted in a buffer containing 50 mM HEPES, pH 7.4, 3 mM MgCl
2
, 3 mM MnCl
2
, 1 mM DTT, 3 ?M Na orthovanadate, 0.1 mM ATP, 30 ?g/ml Poly (Glu:Tyr)4:1 substrate, and 4 ?g /ml recombinant cKit. Assay was done with 0.2 mg/ml Poly-(Glu-Tyr) peptide using ADPGlo
?
(
Promega
) conditions at 30°C for 35 minutes.




Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole




Format:
Aqueous buffer solution




Storage / St
ABI
lity:
>6 months at –80°C.




Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.




Reference(s):
1. Schumaker, J.A., et al. (2008) J. Clin. Pathol. 61:109-114.
2. Sun, J. et al. (2009) J. Biol. Chem. 284: 11039-11047.




Warning(s):
Avoid freeze/thaw cycles




Scientific Category:
Kinase/Receptor Tyrosine




Product Type:
Recombinant Protein




Data shown is lot-specific.
Contact us
for specific information on other lots.