商品编号


50652



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Demethylase(s)



Size

96 reactions

商品信息













Description:
The TET2 Chemiluminescent Assay Kit is designed to measure TET2 activity for screening and profiling applications. TET2 belongs to the Ten Eleven Translocation (TET) family proteins that catalyze 5-methylcytosine oxidation and generate 5-methylcytosine derivatives, including 5-hydroxymethylcytosine. TET2 is one of the most frequently mutated genes in myeloid malignancies.

The key to the TET2 Chemiluminescent Assay Kit is a highly specific antibody that recognizes hydroxymethylated substrate. With this kit, only three simple steps on a microtiter plate are required for detection of TET2 activity. First, TET2 enzyme is incubated with the methylated substrate for two hours. Then the primary antibody is added. Next, the plate is treated with an HRP-labeled secondary antibody. Finally, the HRP substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader.




Synonym(s):
Ten-eleven translocation 2 protein, methylcytosine dioxygenase TET2




Supplied As:
The TET2 Chemiluminescent Assay Kit comes in a convenient format; a 96-well strip plate precoated with methylated DNA substrate, primary antibody, a secondary HRP-labeled antibody, TET2 assay buffer, and purified TET2 for 100 enzyme reactions.




Format:

COMPONENTS:





Instructions for use:
See assay kit data sheet for detailed protocol.




Storage / St
ABI
lity:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.




Reference(s):
Li Tan, Yujiang Geno Shi. Development 2012; 139(11): 1895-1902.




Warning(s):
Avoid freeze/thaw cycle




Scientific Category:
DNA Demethylase




Product Type:
Assay Kit




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