商品编号


50515



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Demethylase(s)



Size

384 reactions

商品信息













Description:
The PHF8 Homogeneous Assay Kit is designed to measure the activity of PHF8 for screening and profiling applications. PHF8, also known as JHDM1F and KDM7B, is a JumonjiC (JmjC) domain-containing histone lysine demethylase that exhibits demethylation activity toward dimethyl-lysine 9 on histone H3. The PHF8 Homogeneous Assay Kit comes in a convenient AlphaLISA? format, with biotinylated histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified PHF8 for 384 enzyme reactions. The key to the PHF8 Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for demethylase activity detection. First, a sample containing PHF8 enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by re
ADI
ng the Alpha-counts.




UniProt
Q9UPP1




Synonym(s):
KDM7B, JHDM1F, PHD finger protein 8, ZNF422




Supplied As:
The PHF8 (KDM7B) Homogeneous Assay Kit comes in a convenient AlphaLISA? format with primary antibody 17-4, FLAG-tagged PHF8, and assay buffers for 384 reactions.




Contraindications:
Green and blue dyes that absorb light in the AlphaScreen signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of culture medium RPMI 1640 at >1% leads to signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen assays.




Format:






Instructions for use:
See assay kit data sheet for detailed protocol




Storage / St
ABI
lity:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.




Application(s):
Great for studying enzyme kinetics and HTS applications.




Reference(s):
Horton J.R.,
et al. Nature Structural & Molecular
BIOLOG
y
2010;
17(1):38-43.




Notes:
AlphaScreen??and AlphaLISA??are registered trademarks of
Perkinelmer
, Inc.




Warning(s):
Avoid freeze/thaw cycles.




Scientific Category:
Demethylase




Product Type:
Assay Kit




Data shown is lot-specific.
Contact us
for specific information on other lots.