商品编号


50614



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Demethylase(s)



Size

384 reactions

商品信息













Description:
The LSD2(KDM1B) Homogeneous Assay Kit (Dimethyl) is designed to measure the activity of lysine-specific demethylase (LSD2) for screening and profiling applications. LSD2, also called KDM1B, is a chromatin-modifying enzyme that specifically removes methyl groups from mono- and dimethylated Lys of histone H3, thereby acting as a corepressor of transcription. LSD2 is a potential target for drug development. The key to the LSD2 Homogeneous Assay Kit (Dimethyl) is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for demethylase detection. First, a sample containing LSD2 enzyme is incubated with the biotinylated substrate. Next, acceptor beads are added, then donor beads, followed by re
ADI
ng the Alpha-counts.




Supplied As:
The LSD2 Homogeneous Assay Kit (Dimethyl) comes in a convenient AlphaLISA? format, with biotinylated, dimethylated histone H3 (2MeK4) peptide substrate, primary antibody to detect mono-methylated product, LSD2 assay buffer, and purified LSD2.




Contraindications:
Green and blue dyes that absorb light in the AlphaScreen signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen assays.




Format:

COMPONENTS:






Storage / St
ABI
lity:
At least one year from date of receipt when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.




Application(s):
Great for studying enzyme kinetics and HTS applications.




Reference(s):
Binda, C., et al., J. Am. Chem. Soc. 2010; 132(19): 6827-6833.
Welte, M., et al., Cell Press. 2005; 15(14): 1266-1275.




Warning(s):
Avoid freeze/thaw cycles.




Scientific Category:
Demethylase




Product Type:
Assay Kit




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