商品编号


50416



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Demethylase(s)



Size

384 reactions

商品信息













Background:
JMJD3 is a JmjC-domain protein that exhibits demethylation activity toward di- and trimethyl-lysine 27 (H3K27me2/3) on histone H3.




Description:
The JMJD3 Homogeneous Assay Kit is designed to measure activity of the JMJD3 for screening and profiling applications, using AlphaLISA
?
technology. The key to the JMJD3 Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JMJD3 enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by re
ADI
ng the Alpha-counts.




Synonym(s):
JMJD3, KDM6B




Supplied As:
Kit comes in a convenient AlphaLISA
?
format, with biotinylated histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified JMJD3 for 384 enzyme reactions.




Contraindications:
Avoid green and blue dyes that absorb light in the Alphascreen
?
signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN
3
) or metal ions (Fe
2+
, Fe
3+
, Cu
2+
, Zn
2+
and Ni
2+
). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect Alphascreen
?
assays.




Format:

COMPONENTS:






Instructions for use:
See assay kit data sheet for detailed protocol.




Storage / St
ABI
lity:

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.




Application(s):
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.




Reference(s):
Swigut T., Wysocka J. Cell 2007; 131(1): 29-32.




Notes:

The buffer in this kit was reformulated in May of 2015 with a different reducing agent to improve assay performance. The old formulation can still be purchased upon special request.

MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
AlphaLISA anti-rIgG acceptor beads, 5 mg/ml (
Perkinelmer
#AL104C)
AlphaScreen Streptavidin-conjugated donor beads, 5 mg/ml (
Perkinelmer
#6760002S)
Optiplate-384 (
Perkinelmer
#6007290)
AlphaScreen microplate reader
Adjustable micropipettor and sterile tips




Warning(s):
Avoid freeze/thaw cycles.




Scientific Category:
Demethylase




Product Type:
Assay Kit




Data shown is lot-specific.
Contact us
for specific information on other lots.