商品编号


50413



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Demethylase(s)



Size

384 reactions

商品信息













Background:
JMJD2A is a JmjC-domain protein that exhibits demethylation activity toward H3-K9Me3 and H3-K36Me3.




Description:
The JMJD2A Homogeneous Assay Kit is designed to measure activity of the JMJD2A for screening and profiling applications, using AlphaLISA
?
technology. The key to the JMJD2A Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JMJD2A enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by re
ADI
ng the Alpha-counts.




Synonym(s):
JMJD2A, JHDM3A, KDM4A




Supplied As:
The JMJD2A Homogeneous Assay Kit comes in a convenient format, with a 384-well plate, biotinylated histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified JMJD2A for 384 enzyme reactions.




Contraindications:
Avoid green and blue dyes that absorb light in the Alphascreen
?
signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN
3
) or metal ions (Fe
2+
, Fe
3+
, Cu
2+
, Zn
2+
and Ni
2+
). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect Alphascreen
?
assays.




Format:

COMPONENTS:





Instructions for use:
See assay kit data sheet for detailed protocol.




Storage / St
ABI
lity:

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.




Application(s):
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.




Reference(s):
1. Whetstine JR et al. (2006) Cell 125: 467.
2. Fodor, B.D., Genes & Dev. 2006. 20: 1557-1562.
APPLICATION REFERENCE:
Nakagawa-Yagi, Y., et al. BMC Complement Altern Med. 12(1):101 (2012).




Notes:

The buffer in this kit was reformulated in May of 2015 with a different reducing agent to improve assay performance. The old formulation can still be purchased upon special request.




Warning(s):
Avoid freeze/thaw cycles.




Scientific Category:
Demethylase




Product Type:
Assay Kit




Data shown is lot-specific.
Contact us
for specific information on other lots.