商品编号


FS-0318-10



品牌


Mabtech



公司


Mabtech



公司分类


Human



Size

10 plates

商品信息
FluoroSpot kit for enumeration of cells secreting IL-17A and IL-22
The FluoroSpot assay is an inventive development of the ELISpot assay and uses fluorescent detection instead of colorimetric. This allows for simultaneous detection of several analytes, which is a major advantage. The human IL-17A/IL-22 FluoroSpot kit enables convenient enumeration of cells secreting either one or both analytes. The kit contains low fluorescent plates, matched quantities of monoclonal capture and detection antibodies, secondary reagents conjugated to fluorophores, a fluorescence enhancer and an anti-CD28 monoclonal antibody for co-stimulation. A polyclonal activator for human T cells (mAb CD3-2) is supplied as a positive control for cytokine secretion.
Human IL-17A/IL-22 FluoroSpot kits are available in 2 or 10-plate format.
For analysis, we recommend the use of an automated FluoroSpot reader equipped with filters for FITC (excitation 490 nm/emission 510 nm) and Cy3 (excitation 550 nm/emission 570 nm). Filters should have high specificity to avoid bleed-through artifacts. Double secreting cells are identified by a computerized overlay.

Analyte Information

IL-17A
Interleukin-17A (IL-17A, IL17A) is a potent proinflammatory cytokine produced by activated Th17 (T-helper 17) cells and certain cells belonging to the innate immune system. In mice, IL-17 has been shown to also be produced by activated CD8+ T cells and γδ T cells. Th17 cells play an important role in autoimmune diseases and protection against bacteria and fungi. IL-17A acts on a broad range of cell types to induce expression of cytokines, chemokines and metalloproteinases. As a result, secretion of IL-17A promotes inflammatory responses, which leads to the recruitment of neutrophils, enhancement of antibody production and activation of T cells. Increased expression of IL-17A is seen in autoimmune diseases such as multiple sclerosis and rheumatoid arthritis. It is also associated with asthma, psoriasis, cancer and transplant rejection.
IL-22
The cytokine Interleukin-22 (IL-22, IL22) is mainly produced by activated CD4+ T-helper 17 (Th17) and T-helper 22 (Th22) cells. The IL-22 receptor is expressed on non-immune cells, particularly epithelial cells and keratinocytes, and IL-22 promotes innate immune responses versus bacterial infections in these target cells. In addition, Th22 cells have been shown to play a role in the pathophysiology of many human skin diseases.
Application:


FluoroSpot


Reactivity:


Human


Contents:


Capture mAb (MT44.6)
Capture mAb (MT12A3)
BAM-conjugated detection mAb (MT504)
Biotinylated detection mAb (MT7B27)
Anti-BAM-490
SA-550
anti-CD3 mAb
Anti-CD28 mAb (CD28-A)
Fluorescence enhancer
10
FluoroSpot plates


Size:

10 plates

Other formats:

Click to view all Human IL-17A, IL-22products