商品编号


SL-3001-NP



品牌


Signosis



公司


Signosis



公司分类


Cell-Based Assays




1 Ea

商品信息

Description:
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Principle:
Nuclear hormone receptors (NHRs) are a group of ligand-binding transcription factors (TFs).
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More than 350 NHRs are available in the PDB. Like other TFs, they are able to regulate gene expression by binding to specific DNA regulatory elements, but their activities are modulated by the corresponding ligands. They play many important physiological functions such as embryonic development, organ physiology, cell differentiation and homeostasis. They are also associated with human diseases including cancer, obesity/diabetes, arthritis and asthma. Members of this family are often chosen to be drug targets.

Conventionally, the response element-luciferase reporter assays are used to study endogenous or exogenous receptors, and analyze the receptor signaling pathway in a native
BIOLOG
ical context.
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Due to their low sensitivity of these assays and the similar response element shared by multiple NHRs, their applications are limited.
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Signosis
has developed LDB-driven GAL4 reporter HEK 293 stable cell lines by delivering two vectors, one expressing 8 copies of GAL4 upstream activator sequences (UAS) in front of the luciferase reporter gene, another expressing Gal4 DNA Binding Domain (D
BD
) and GR ligand binding domain(L
BD
). Upon addition of a corresponding ligand, the D
BD
-L
BD
fusion protein is activated, and binds to GAL promoter binding site, le
ADI
ng to the induction of luciferase.
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The stable clones were selected with hygromycin and G418, The functional assay was subsequently conducted by DEX treatment, and the clone with the highest induction fold (50) has been chosen for this product. The advantages of these stable cell lines are low cross-reactivity with other nuclear receptors and less toxicity of the chimeric receptors to the cells when overexpressed.
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These cell lines can be used to screen drug compounds for ago
NIST
or antago
NIST
hit identification.

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