商品编号


FS-05R17G-2



品牌


Mabtech



公司


Mabtech



公司分类


Human/NHP



Size

2 plates

商品信息
B-cell FluoroSpot kit for enumeration of cells secreting human IgG/IgM
The FluoroSpot assay is an inventive development of the ELISpot assay and uses fluorescent detection instead of colorimetric. This allows for simultaneous detection of several analytes. The human IgG/IgM B-cell FluoroSpot kit enables detection of IgG- and IgM-secreting cells in the same well.
The kit contains matched quantities of capture antibodies and fluorophore-conjugated detection antibodies, low fluorescent PVDF plates and a fluorescence enhancer. The polyclonal activators R848 and recombinant human IL-2 are included for pre-stimulation of memory B cells.
For analysis, we recommend the use of an automated FluoroSpot reader equipped with filters for FITC (excitation 490 nm/emission 510 nm) and Cy3 (excitation 550 nm/emission 570 nm). Filters should have high specificity to avoid bleed-through artifacts.
Species cross-reactivity
The antibodies in this kit cross-react with IgG and IgM from non-human primates. See the Human/NHP cross-reactivity table.

Analyte Information

IgG
IgG (Immunoglobulin G) is the most abundant Ig isotype in serum and makes up approximately 80% of all serum Immunoglobulins. In humans, there are four subclasses of IgG with highest serum concentrations of IgG1 followed by IgG2, IgG3 and IgG4. In mice the IgG subclasses are defined IgG1, IgG2a/c, IgG2b and IgG3. The IgG molecule consists of two heavy and two lights chains (κ or λ) resulting in a molecule with two arms for antigen binding. High levels of IgG antibodies are induced following the initial IgM response in a typical immune response to antigens.
IgM
Monomeric IgM (Immunoglobulin M) is found on the surface of B cells whereas the IgM molecules secreted from plasma B cells are pentamers, i.e. five IgM molecules joined together by the J chain. IgM constitutes approximately 10% of the total Ig concentration in serum. IgM is the first antibody isotype induced in a typical immune response and hence is the isotype with the lowest affinity for antigens. Due to its structure, with 10 antigen-binding sites per IgM pentamer, it can nevertheless bind e.g. surface-bound antigens with a very high
Avidity
.
Application:


FluoroSpot


Reactivity:


Human / NHP




Contents:


Capture mAbs (MT91/145)
Capture mAbs (MT11/12)
Detection mAbs anti-human IgG-550 (MT78/145)
Detection mAb anti-human IgM-490 (MT22)
R848
Recombinant human IL-2
Fluorescence enhancer
2
FluoroSpot plates


Size:

2 plates

Other formats:

Click to view all Human/NHP IgG, IgMproducts