商品编号


SL-0040-FP



品牌


Signosis



公司


Signosis



公司分类


Cell-Based Assays




1 Ea


商品信息

Description:
ELK Responsive Luciferase Reporter HEK293 Cell Line?is derived human embryonic kidney, and stably express firefly luciferase reporter gene under the control of ELK response element. This cell line is an ideal cellular model for monitoring the activation of MAPK response Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.
Principle:
Mitogen-activated protein kinase (MAPK) signaling pathways mediate important eukaryotic transcriptional responses to extracellular signals that begin at the cell surface.? While different external stimuli may activate distinct MAPK signaling pathways, many MAPK pathways later converge in the activation of ETS domain transcription factor ELK1.? ELK1 is a major nuclear substrate for MAPKs, JNK, p38, and ERK pathways, where phosphorylation of ELK1 by these kinases results in a conformational change of ELK1 and triggers its DNA binding activity.?

Signosis
has established an ELK-TAD luciferase reporter stable cell line.? This cell line expresses a fusion protein linking the DNA transactivation domain (TAD) of ELK to Gal4.? As the TAD is phosphorylated, this Gal4 fusion protein will drive luciferase expression controlled by Gal4-UAS.? This cell line can act as a reporter system to monitor ELK1-dependent transcriptional activity and MAPK signaling, as triggered by stimuli treatment, enforced gene expression and gene knockdown.

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Data:

Analysis of ELK-TAD Reporter Cell Line in response to MEKK overexpression.?
The cells were transfected with varying concentration of MEKK.? 48 hours after transfection, cell lysate was collected to measure the luciferase activity.? ELK-TAD reporter activity showed a gene dose-dependent increase to MEKK overexpression.