商品编号


FS-0103-10



品牌


Mabtech



公司


Mabtech



公司分类


Human



Size

10 plates














商品信息
FluoroSpot kit for enumeration of cells secreting IFN-γ and IL-17A
The FluoroSpot assay is an inventive development of the ELISpot assay and uses fluorescent detection instead of colorimetric. This allows for simultaneous detection of several analytes, which is a major advantage. The human IFN-γ/IL-17A FluoroSpot kit enables convenient enumeration of cells secreting either one or both analytes. The kit contains low fluorescent plates, matched quantities of monoclonal capture and detection antibodies, secondary reagents conjugated to fluorophores, a fluorescence enhancer and an anti-CD28 monoclonal antibody for co-stimulation. A polyclonal activator for human T cells (mAb CD3-2) is supplied as a positive control for cytokine secretion.
Human IFN-γ/IL-17A FluoroSpot kits are available in 2 or 10-plate format.
For analysis, we recommend the use of an automated FluoroSpot reader equipped with filters for FITC (excitation 490 nm/emission 510 nm) and Cy3 (excitation 550 nm/emission 570 nm). Filters should have high specificity to avoid bleed-through artifacts. Double secreting cells are identified by a computerized overlay.

Analyte Information

IFN-γ
Interferon-γ (IFN-γ, also known as IFN-gamma, IFN-g, IFNg and Interferon-gamma) is produced mainly by activated T cells and NK cells. It is a proinflammatory cytokine that activates macrophages and endothelial cells, but it also regulates immune responses by effecting APC and T and B cells. Production of IFN-γ by helper T cells as well as cytotoxic T cells is a hallmark of the TH1-type phenotype, thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.
IL-17A
Interleukin-17A (IL-17A, IL17A) is a potent proinflammatory cytokine produced by activated Th17 (T-helper 17) cells and certain cells belonging to the innate immune system. In mice, IL-17 has been shown to also be produced by activated CD8+ T cells and γδ T cells. Th17 cells play an important role in autoimmune diseases and protection against bacteria and fungi. IL-17A acts on a broad range of cell types to induce expression of cytokines, chemokines and metalloproteinases. As a result, secretion of IL-17A promotes inflammatory responses, which leads to the recruitment of neutrophils, enhancement of antibody production and activation of T cells. Increased expression of IL-17A is seen in autoimmune diseases such as multiple sclerosis and rheumatoid arthritis. It is also associated with asthma, psoriasis, cancer and transplant rejection.
Application:


FluoroSpot


Reactivity:


Human


Contents:


Capture mAb (1-D1K)
Capture mAb (MT44.6)
Biotinylated detection mAb (MT504)
FITC-conjugated detection mAb (7-B6-1)
Anti-FITC-490
SA-550
anti-CD3 mAb
Anti-CD28 mAb (CD28-A)
Fluorescence enhancer
10
FluoroSpot plates


Size:

10 plates

Other formats:

Click to view all Human IFN-γ, IL-17Aproducts





We have used
Mabtech
ELISPOT antibodies and reagents for several years. The high quality and standard and competitive price
will keep us hooked for years to come
. Recently we have started working with Fluorospot, which has really helped us move forward and have opened new avenues in our research. As always management and technical staff have all provided impeccable service.







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Mabtech/Human IFN-γ/IL-17A FluoroSpot kit/FS-0103-10/10 plates






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