商品编号


M7540-mt



品牌


Sciencell



公司


Sciencell research lab



公司分类


Human Pluripotent Stem Cells




1 Ea

商品信息
Isolated from E14 mouse embryos. These cells are cryopreserved at P3 after mitomycin C treatment and delivered frozen. Each vial contains 1 x 10^6 cells in 1 ml volume.

Mouse Embryonic Fibroblasts (MEF) are used to support the growth of undifferentiated mouse or human ES and iPS cells. They provide both a substrate for the ES cells to grow on and secrete many factors necessary for ES cells to maintain pluripotency. MEF cells are isolated from mouse embryos and are used at their early passages. These cells have been mitomycin treated to prevent further cell division prior to cryopreservation.

MEF cells from
Sciencell
Research Laboratories are isolated from E14 mouse embryos. These cells are cryopreserved at P3 after mitomycin C treatment and delivered frozen. Each vial contains 1 x 10^6 cells in 1 ml volume. MEF-mt are characterized by immunofluorescent imaging using antibodies to fibronectin. MEF-mt are negative for mycoplasma, bacteria, yeast and fungi.

Recommended Medium

It is recommended to use DMEM (Cat. #09221) supplemented with 10% fetal bovine serum (FBS, Cat. #0010, 0025, 0500) for culturing MEF-mt in vitro.

Specifications:
Catalog No.
M7540-mt
Country of Manufacture
United States
Product Code
MEF-mt
Size/Quantity
1 x 10^6 cells/vial
Product Use
MEF are used as a feeder layer in mouse and human pluripotent stem cell culture. They are forresearch use only. They are not approved for human or animal use, or for application in in vitrodiagnostic procedures.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen andkeep the cells in liquid nitrogen until they are needed for experiments.
Shipping Info
Dry ice.
References
[1] BRADLEY, A. (1987). Production and analysis of chimaeras. In Teratocarcinomas and Embryonic Stem Cells: A Practical Approach, (ed. E. J. Robertson), pp. 113-151. Oxford: IRL Press. [2] Nagy et al. (2006) Preparing Mouse Embryo Fibroblasts Cold Spring Harbor Protocols. 2006: pdb.prot 4398.