商品编号


M1920-57



品牌


Sciencell



公司


Sciencell research lab



公司分类


Hematopoietic Cell System




1 Ea

商品信息
Isolated from adult C57BL/6 mouse bone marrow. Cells are harvested after purification and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume.

Macrophages are cells differentiated from circulating bone marrow-derived monocytes. In the bone marrow and subsequestly in the blood and tissues, monocytes undergo a cascade of maturation that culminate in morphologically and functionally mature tissue macrophages [1]. Macrophages function to removal cellular debris and inv
ADI
ng pathogens. They phagocytize inv
ADI
ng microorganisms, and scavenge dead, damaged cells and cellular debris. Macrophages can be identified by the specific expression of several cell surface proteins including CD14, CD11b, F4/80 (mice)/EMR1 (human), MAC-1/MAC-3, and CD68 by flow cytometry or immunohistochemical staining [2].

MMa-bm from
Sciencell
Research Laboratories are isolated from adult mouse bone marrow. MMa-bm are harvested at P0 and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. MMa-bm are characterized by immunofluorescence with antibody specific to CD11b and F4/80.?MMa-bm are negative for mycoplasma, bacteria, yeast, and fungi. MMa-bm are guaranteed to further culture under the conditions provided by
Sciencell
Research Laboratories; however,
MMa-bm are not recommended for expanding or long-term cultures since the cells do not proliferate in regular culture.

Recommended Medium

It is recommended to use Macrophage Medium (MaM, Cat. #1921) for the culturing of MMa-bm
in vitro
.

Specifications:
Catalog No.
M1920-57
Country of Manufacture
United States
Product Code
MMa-bm
Size/Quantity
1 x 10^6 cells/vial
Product Use
MMa-bm are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.
Shipping Info
Dry ice.
References
[1]. Cline MJ and Sumner MA. (1972) Bone Marrow Macrophage Precursors. I. Some Functional Characteristics of the Early Cells of the Mouse Macrophage Series. Blood. 40: 62-69. [2]. Wynn TA and Barron L. (2010) Macrophages: master regulators of inflammation and fibrosis. Semin Liver Dis. 30: 245-57. [3]. Gordon S and Taylor RP. (2005) Monocyte and macrophage heterogeneity. Nature Reviews Immunology. 5: 953-964.