商品编号


ax4012



品牌


Axolbio



公司


Axolbio



公司分类


Oncology




1 Ea

商品信息
Tamoxifen-Resistant MCF7 Cells (MCF7/TAMR-8) (1 million cells)
Description:
MCF7/TAMR-4 and MCF7/TAMR-8 are tamoxifen-resistant MCF7 breast cancer cell lines. The parental MCF7/S0.5 line was treated with 1 μM tamoxifen for 28 days which killed the majority of the cells. The surviving cells were clonally expanded as sublines in the absence of tamoxifen for 19 passages and were then treated continuously with 1 μM tamoxifen. The resistant cells continued to proliferate and approached a growth rate that was comparable to the parental MCF7/S0.5 line.
Condition of sale: the end user is not permitted to transfer or re-freeze the cells.
Product Specification:


Donor gender

Female



Donor age at sampling

69 yrs



Genetic modification

None



Size

1 million cells



Growth properties

Adherent



Disease information

Malignant adenocarcinoma derived from pleural?effusion



General notes

Expresses the estrogen receptor (ER); resistant to tamoxifen at 1 μM



Shipping conditions

Dry ice



Storage conditions

Liquid nitrogen



Usage notes

Culture in DMEM/F12 medium (no phenol red) supplemented with 1% fetal bovine serum + 2.5 mM GlutaMAX + 6 ng/mL insulin. Addition of 1 μM tamoxifen is required to maintain a high level of resistance



Condition of sale

The end user is not permitted to transfer or re-freeze the cells