商品编号


4822-96-K



品牌


Trevigen



公司


Trevigen, Inc



公司分类


Apoptosis



Size

96 Samples

商品信息

Description

During the process of apoptosis, DNA fragmentation occurs following the activation of endonucleases. The labeling of the 3’ ends of DNA fragments provides an easy measure of cells undergoing apoptosis. Modified nucleotides are incorporated at the 3’ ends by the activity of terminal deoxynucleotidyl transferase (TdT). These nucleotides are detected using a horser
ADI
sh-peroxidase detection system and TACS-Sapphire?. TiterTACS? can be used with s
USP
ension or monolayer cells. The kit is designed to use fixed samples, allowing you to work safely with samples that are infected with biohazardous agents. Fixed samples may be stored conveniently during time-course experiments. The TiterTACS? Kit also provides TACS-Nuclease? solution to generate positive controls from your own samples giving you a maximal value for the assay.

Detection using TACS-Sapphire?, a non-toxic colorimetric substrate, allows both kinetic and endpoint re
ADI
ngs. The reaction generates a blue product that can be measured at 630 nm, and will turn yellow after the reaction is stopped with acid allowing endpoint re
ADI
ng at 450 nm.

FEATURES:


Convenient, 96 well microplate format.

Quantitative colorimetric detection.

Includes both Proteinase K and exclusive Cytonin? non-enzymatic perme
ABI
lization reagents.

Includes TACS-Nuclease solution for preparing sample-dependent positive controls.


APPLICATIONS:

Identification and quantitation of apoptosis in cultured cells in a 96 well assay.




Catalog # 4822-96-K includes :



Catalog Number

Description

Qty



4800-30-01

Proteinase K

2



4800-30-06

Streptavidin-HRP

1



4800-30-12

Blue Strep-HRP Diluent

1



4800-30-15

TACS-Nuclease?

1



4800-30-16

TACS-Nuclease? Buffer

1



4810-30-04

TACS 2 TdT dNTP

1



4810-30-14

50x Manganese Cation

2



4817-60-02

10X TdT Labeling Buffer

1



4817-60-03

10X TdT Stop Buffer

1



4821-96-05

TdT Enzyme

1



4822-96-08

TACS-Sapphire?

1



4876-05-01

Cytonin?

1




?

“ADAM12: a genetic modifier of preclinical peripheral arterial disease”
“Ayotunde O. Dokun, Lingdan Chen, Mitsuharu Okutsu, Charles R. Farber, Surovi Hazarika, W. Schuyler
Jones, Damian Craig, Douglas A. Marchuk, R. John Lye, Svati H. Shah, and Brian H. Annex”
Am J Physiol Heart Circ Physiol, Sep 2015; 309: H790 – H803.
http://ajpheart.physiology.org/cgi/content/abstract/309/5/H790

“Desensitization of idiopathic pulmonary fibrosis fibroblasts to Alternaria alternata extract-mediated necrotic cell death”
Jintaek Im, Kyutae Kim, Ji Young Yhee, Scott M. O’Grady, and Richard S. Nho PHY2, Nov 2016; 4: e13020.
http://physreports.physiology.org/cgi/content/abstract/4/21/e13020