商品编号


U1130



品牌


UBPBio



公司


Ubiquitin-Proteasome Biotechnologies, LLC



公司分类


Apoptosis / Necrosis



Size

50 ?g


商品信息

TNFalpha (Tumor necrosis factor alpha) is produced by immune and epithelial cells. It plays important roles in regulating inflammation, apoptosis, and immune response. It exerts its function mainly by binding of two cell surface receptors TNFR1 and TNFR2. Treating cells with TNFalpha activates the NFkappaB, MAPK, and apoptotic signaling pathways. The recombinant GST-TNFalpha protein consists of a N-terminal GT tag with the C-terminal extracellular domain (amino acids 77-233) of full length TNFalpha. It can be used for pulldown assays. It binds TNFR1 and TNFR2 to induce downstream signaling pathways, but often requires higher concentrations than TNFalpha (catalog # U1120).


Additional Information






Product Name:

GST-TNFalpha



Also Known As:

TNF; DIF; TNFA; TNFSF2; TNF-alpha



Catalog No.:

U1130



Size:

50 ?g



Molecular Weight:

25.6 kDa



Species:

Human



Source:

Bacterial recombinant



Stock:

20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol



Concentration:

See tube label



Quality Assurance:

~ 85% by SDS-PAGE



Storage:

Store at -80°C; avoid multiple freeze-thaw cycles



PDF Data Sheet:

PDF Datasheet



NCBI RefSeq:

NM_000594.3



Image(s):

(Click image to enlarge)








Coomassie-stained SDS-PAGE
Lane 1: Molecular weight
Marker
s
Lane 2: 5 ?g purified TNFalpha







Shipping Method:

Dry ice shipping



References:

N/A




Details

TNFalpha (Tumor necrosis factor alpha) is produced by immune and epithelial cells. It plays important roles in regulating inflammation, apoptosis, and immune response. It exerts its function mainly by binding of two cell surface receptors TNFR1 and TNFR2. Treating cells with TNFalpha activates the NFkappaB, MAPK, and apoptotic signaling pathways. The recombinant GST-TNFalpha protein consists of a N-terminal GT tag with the C-terminal extracellular domain (amino acids 77-233) of full length TNFalpha. It can be used for pulldown assays. It binds TNFR1 and TNFR2 to induce downstream signaling pathways, but often requires higher concentrations than TNFalpha (catalog # U1120).

Images:
(Click image to enlarge)








Coomassie-stained SDS-PAGE
Lane 1: Molecular weight
Marker
s
Lane 2: 5 ?g purified TNFalpha