商品编号


A14525



品牌


Gibco



公司


Thermo Fisher Scientific



公司分类


Transfection Reagents



Quantity

For 10 L of culture

商品信息

Description
The ExpiFectamine? 293 Transfection Kit is a core component of the Expi293? Expression System. It is designed for transient transfection of high density cultures of HEK (human embryonic kidney) 293 cells. The high-efficiency, cationic, lipid-based transfection reagent and transfection enhancers are designed to power the highest poss
IBL
e level of protein expression from Expi293F? cells cultured in Expi293? Expression Medium
?
Designed
specifically for transfection of high density s
USP
ension cell culture, with matching transfection enhancers that boost transfection performance and protein expression
?
Achieves
protein yields 2- to 10-fold higher than other transfection reagents used on high density 293 cell cultures
?
Employs
the same transient expression protocols typically used in current low-density 293 s
USP
ension culture systems to easily switch from low density systems to the high yield, high density Expi293? Expression System
?
Provides
robust and reproduc
IBL
e transfection results, giving users greater confidence in their results
?
Scale
transfections for culture volumes of less than 1 mL to greater than 10 liters, while maintaining equivalent volumetric protein yields
The ExpiFectamine? 293 Transfection Kit is specifically designed to work with Expi293F? cells in the Expi293? Expression System. The transfection kit consists of the cationic lipid-based ExpiFectamine? 293 Reagent and ExpiFectamine? 293 Transfection Enhancers 1 and 2. The enhancers are added 16 to 18 hours post-transfection to boost transfection, cell vi
ABI
lity, and protein expression. The protocol for use of ExpiFectamine? 293 Transfection components is very similar to transfection protocols for other transient expression reagents, such as 293fectin and FreeStyle? MAX.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell Type:
Established Cell Lines
Sample Type (Specific):
Plasmid DNA
Classification:
Animal Origin-Free,?Chemically Defined,?Protein-Free,?Serum-Free
Transfection Technique:
Lipid-Based Transfection
Serum Compat
IBL
e:
No
Cell Line:
Expi293F?
Product Line:
ExpiFectamine?
Product Size:
For 1 L of culture
Purity or Quality Grade:
Cell Culture Grade
Contents & storage
2.7 mL (3 x 0.9 mL) ExpiFectamine? 293 Reagent
5 mL ExpiFectamine? 293 Transfection Enhancer 1
50 mL ExpiFectamine? 293 Transfection Enhancer 2
Sufficient for transfection of 1 L of culture
Store at +2°C to +8°C