商品编号


15338500



品牌


Gibco



公司


Thermo Fisher Scientific



公司分类


Transfection Reagents



Quantity

15 ml













商品信息

Description
Lipofectamine? LTX Reagent is a plasmid transfection reagent that offers the right balance of potency and gentleness for your cells, resulting in the highest poss
IBL
e transfection efficiencies and vi
ABI
lities. Lipofectamine? LTX Reagent is a new, advanced solution for gene expression in both common and challenging cell types. No other plasmid DNA-specific transfection reagent can match the efficiency, convenience, and gentleness of Lipofectamine? LTX Reagent for transfection of primary, challenging, and sensitive cell lines. Lipofectamine? LTX Reagent is synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cell lines. A certificate of origin may be obtained by contacting Technical Support.
Lipofectamine? LTX Reagent offers:
? Superior delivery – transfect a broad range of cell types, including primary challenging and sensitive cells, with high efficiency
? Superior performance – combine high transfection efficiency with maximal (>90%) cell vi
ABI
lity
? Optimized protocols – rapid, simple protocols provided for most cell lines
? A highly effective reagent to generate stable cell lines
Transfect Primary Cells Efficiently
Primary cells are often difficult to transfect. Lipofectamine? LTX Reagent, when combined with PLUS? Reagent, provides the highest level of transfection in primary cells of any lipid- or polymer-based reagent. Many primary cell types have been successfully transfected with Lipofectamine? LTX including primary neural
Progen
itor cells.
Superior Delivery into Challenging Cells
When it comes to achieving high levels of protein expression in RAW267.3 macrophages, Lipofectamine? LTX Reagent outperforms competing transfection reagents (see Figure 4). Achieving high-level transgene expression in cells that are tr
ADI
tionally refractory to transfection allows you to obtain results that were previously unattainable.
Broad Compatibility with Cell Lines
In addition to being excellent for transfecting primary and challenging cells, Lipofectamine? LTX Reagent provides you with the most versatile approach to transfecting established cells lines, including those that are sensitive to other transfection reagents. Use our Transfection Selection Tool to help you select the best transfection conditions for the cells with which you work.
Stable Transfection
Lipofectamine? LTX strikes the right balance between potency and gentleness, giving you the highest poss
IBL
e percentage of successfully transfected cells. This makes Lipofectamine? LTX the quickest and easiest reagent for establishing stable transfectants from challenging or refractory cell types.
Simple, Rapid, Serum-compat
IBL
e Protocol
Lipofectamine? LTX Reagent offers a streamlined protocol – no need to remove transfection complexes or change/add medium following transfection. A simple, optimization protocol helps you get the best results quickly so you'll spend less hands-on time, and get faster results.
Lipofectamine? LTX and PLUS? Reagent is available in the following sizes:
? 0.1 ml with 40 ?l PLUS? Reagent
? 0.3 ml with 0.25 ml PLUS? Reagent
? 1.0 ml with 0.85 ml PLUS? Reagent
? 15 ml (purchase PLUS? Reagent separately)
For Research Use Only. Not for use in diagnostic procedures.
Figures
Lipofectamine?? LTX transfection efficiency
Lipofectamine?? LTX Reagent combines high transfection efficiency with low cytotoxicity superior to FuGENE?? 6 and FuGENE?? HD transfection reagents
Transfection optimization of Human Mesenchymal Stem Cells (hMCS)
Plasmid transfection.
Lipofectamine?? LTX in the presence of PLUS??? Reagent is an excellent choice for transfection of human cancer cell lines as well as primary and stem cells. The reagent enables the delivery ofhigh transfection performance without associated cytotoxicity
Lipofectamine?? LTX Reagent, with Plus??? Reagent, efficiently transfects primary neural
Progen
itor cells
A simple, rapid, serum-compat
IBL
e protocol
Lipofectamine?? LTX transfection protocol.
Lipofectamine?? LTX Reagent, with PLUS??? Reagent, efficiently transfects primary neural
Progen
itor cells
Lipofectamine?? LTX Transfection Procedure Details
Lipofectamine?? LTX Transfection Workflow
Specifications
Cell Type:
Established Cell Lines,?Hard-to-Transfect Cells,?Primary Cells,?Stem Cells
Sample Type (Specific):
Plasmid DNA,?RNAi Plasmids (shRNA, miR)
Classification:
Animal Origin-Free
Transfection Technique:
Lipid-Based Transfection
Serum Compat
IBL
e:
Yes
High Throughput Compatibility:
High Throughput-Compat
IBL
e
Product Line:
Lipofectamine?
Product Size:
15 ml
Shipping Condition:
Wet Ice
Contents & storage
Each unit contains one vial of Lipofectamine? LTX Reagent (15 mL). Store at 2–8°C. Guaranteed stable for 6 months when properly stored.