商品编号


L3000150



品牌


Gibco



公司


Thermo Fisher Scientific



公司分类


Transfection Reagents



Quantity

15 ml








商品信息

Description
Lipofectamine? 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproduc
IBL
e results.This reagent delivers superior transfection efficiency and improved cell vi
ABI
lity for the widest range of hard-to-transfect and common cells (e.g., HEK293, HeLa).
Successfully transfect the widest variety of
BIOLOG
ically relevant cell types with a reagent that offers:
? Superior performance—our highest efficiency reagent for difficult-to-transfect cells
? Improved cell vi
ABI
lity—gentle on your cells, with low toxicity
? Versatile—one reagent for DNA, RNA, and co-transfection

Efficiently Transfect Difficult-to-Transfect Cells
Lipofectamine? 3000 reagent is designed to efficiently transfect difficult-to-transfect cells, yielding superior transfection performance across the broadest array of cell types.Successfully transfected cell types include:
Fibroblast (3T3, COS-7)
Myoblast (C2C12, L6 CRL-1458)
Hepatocyte (HepG2, HuH7)
Erythroleukemic cell (K562)
Breast carcinoma (MCF7, Hs578T)
Prostate carcinoma (LNCap)
Lung carcinoma (A549, NCI-H460)
Osteosarcoma (U-2 OS, Saos-2)
Colon carcinoma (Caco2, SW480)
Pancreatic carcinoma (PANC-1)
Skin melanoma (SK-MEL-28)
?
View performance data for 60+ cell lines plus comparison to Lipofectamine 2000 >
Transfect Common Cells Efficiently and Cost-Effectively
For common cell types, Lipofectamine? 3000 reagent is more cost-effective than other reagents due to its high concentration and low usage amount. The 1.5 mL size provides up to 1500 transfection reactions (in 24-well plates).
Achieve Superior Transfection Efficiency While Maintaining Low Toxicity
Lipofectamine? 3000 reagent maintains a high transfection efficiency within a robust dynamic range of lipid doses for quick and easy optimization. A low toxicity lipid dose (0.75 ?L for 24-well plates) is suggested for applications requiring minimal disruption of the cells.
Enable High Transfection Efficiency in Novel Genome Editing Applications
Lipofectamine? 3000 reagent is suitable for novel genome editing applications. It increases the likelihood of successful cleavage and recombination with TALENs or CRISPRs through highly efficient transfection, ultimately maximizing the efficiency of genetic modifications and simplifying the downstream processes.
For Research Use Only. Not for use in diagnostic procedures.
Figures
Part 1: Better performance in an array of cell lines compared to Lipofectamine?? 2000 reagent
Lipofectamine?? 3000 transfection procedure details
Lipofectamine?? 3000 transfection workflow
Enhanced transfection efficiency using Lipofectamine?? 3000
Part 2: Better performance in an array of cell lines compared to Lipofectamine?? 2000 reagent
Cleavage efficiency for GeneArt?? TALENs and CRISPRs
Specifications
Cell Type:
Established Cell Lines,?Hard-to-Transfect Cells,?Primary Cells,?Stem Cells
Sample Type (Specific):
Plasmid DNA,?RNAi Plasmids (shRNA, miR),?Synthetic siRNA
Classification:
Animal Origin-Free
Transfection Technique:
Lipid-Based Transfection
Serum Compat
IBL
e:
Yes
Product Line:
Lipofectamine?
Product Size:
0.1 mL
Contents & storage
Contains one vial of Lipofectamine? 3000 Reagent and one vial of P3000TM Enhancer Reagent. Store at 2–8°C. Do not freeze.