商品编号


8298



品牌


Sciencell



公司


Sciencell research lab



公司分类


Mesenchymal Stem Cells




1 Ea

商品信息
Four qPCR controls are included in this kit to verify successful reverse transcription of messenger RNA (mRNA) to complementary DNA (
CDN
A), reveal the presence of genomic DNA (gDNA) contamination in
CDN
A samples, and detect qPCR inhibitor contamination.

Human mesenchymal stem cells (MSCs) are a population of multipotent cells that can be differentiated into multiple lineage-specific cells, which can form bone, fat, cartilage, muscle and tendon. Among them,
ADI
pocytes are one of the cell types that can be derived from MSCs through
ADI
pogen
esis. The process of fat formation plays a role in obesity, cardiovascular diseases and metabolic disorders.
Sciencell
has created a convenient qPCR kit for the assessment of human mesenchymal stem cell
ADI
pogenesis. CEBPB, PPARG and FABP4 qPCR primers included in the kit allow for the detection and quantification of early-, mid- and late-stage human MSC
ADI
pogenesis, respectively.

Note
:
all gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

Four qPCR controls are included in this kit to verify successful reverse transcription of messenger RNA (mRNA) to complementary DNA (
CDN
A), reveal the presence of genomic DNA (gDNA) contamination in
CDN
A samples, and detect qPCR inhibitor contamination. Good quality
CDN
A is a critical component for successful gene expression analysis.

Each primer set included in HMSC-A-qPCR kit arrives lyophilized in a 2 mL vial. All primers are designed and tested under the same parameters: (i) an optimal annealing temperature of 65°C (with 2 mM Mg2+, and no DMSO); (ii) recognition of all known target gene transcript variants; and (iii) specific amplification of only one amplicon. Each primer set has been validated by qPCR by melt curve analysis and gel electrophoresis.

Specifications:
Catalog No.
8298
Country of Manufacture
United States
Product Code
HMSC-A-qPCR
Size/Quantity
50 reactions
Product Use
HMSC-A PCR kit is for research use only. Not for use in animals, humans, or diagnostic procedures.
Storage
Store at -20°C upon receipt. Avoid repeated freeze thaw cycles by making six aliquots at 110 μL each.
Shipping Info
Dry ice.
References
[1] Menssen, A. et al. (2011). Differential gene expression profiling of human bone marrow-derived mesenchymal stem cells during
ADI
pogenic development. BMC Genomics. 12: 461-477.