商品编号


5881



品牌


Sciencell



公司


Sciencell research lab



公司分类


Human Pluripotent Stem Cells




1 Ea

商品信息
MEF-cm consists of 100 mL of the medium in two 50-mL bottles.

Mouse Embryonic Fibroblast-Conditioned Medium (MEF-cm) is a serum free medium for the feeder independent culture of human pluripotent stem cells (hPSCs), including embryonic stem cells and induced pluripotent stem cells. MEF-cm is an efficient alternative to MEF feeder cells, supporting the self-renewal of hPSC and maintaining pluripotency in the presence of basic FGF and hPSC qualified matrix (e.g.,
BD
MatrigelTM matrix).

Sciencell
’s MEF-cm was prepared using high quality mitomycin C treated MEF (MEF-mt, Cat. #M7570-mt) derived from E13 embryos of CF1 mice. Serum free medium was conditioned with mitomycin C treated CF1 MEFs for 24 hours. The conditioned medium was collected daily for 7 days, pooled and sterilized by filtering through a 0.22 ?m filter.

Before using MEF-cm for hPSC culture, please add fresh recombinant human basic FGF (4 ng/mL) (rhbFGF, Cat. #104-02) into the medium to make the complete medium. The complete MEF-cm has been tested for its
ABI
lity to support hPSC self-renewal on
BD
MatrigelTM matrix in vitro. The medium color of each lot may vary but does not affect performance.

Additional Materials Required

The recombinant human basic FGF (rhbFGF, Cat. #104-02) should be purchased separately.

Specifications:
Catalog No.
5881
Country of Manufacture
United States
Product Code
MEF-cm
Size/Quantity
100 ml
Product Use
MEF-cm is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Store MEF-cm at ≤ -20°C in a manual defrost freezer. Thaw the medium overnight at 2-8°C in the dark. Aliquot and store the unused portions at ≤ -°C. Long-term storage (>2 weeks) at 2-8°C is not recommended. Avoid repeated freeze-thaw cycles.
Shipping Info
Dry Ice