商品编号


5340



品牌


Sciencell



公司


Sciencell research lab



公司分类


Hematopoietic Cell System




1 Ea

商品信息
HHMa from ScienCell Research Laboratories are isolated from human liver. HHMa are cryopreserved after purification and delivered frozen. Each vial contains > 1 x 10^6 cells in 1 ml volume.

Macrophages are cells differentiated from circulating bone marrow-derived monocytes. The main function of macrophages is to remove cellular debris and destroy inv
ADI
ng pathogens. Human Hepatic Macrophages (HHMa), which are also known as Kupffer cells, reside within the lumen of liver sinusoids. HHMa protect the liver by responding to pathogens and metastatic cells, while tolerating harmless self and foreign antigens, which enter via blood flow through the portal vein and hepatic artery [1]. Recent studies have shown that hepatic macrophages play an important role in fibrosis, liver inflammation, fatty liver disease, and liver transplantation [2-4]. HHMa are an excellent model for studying macrophage functions under normal physiological and pathological conditions.

HHMa from
Sciencell
Research Laboratories are isolated from human liver. HHMa are cryopreserved after purification and delivered frozen. Each vial contains > 1 x 10^6 cells in 1 ml volume. HHMa are characterized by immunofluorescence with antibody to F4/80. HHMa are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HHMa are guaranteed to further culture in the conditions provided by
Sciencell
Research Laboratories; however,
HHMa are not recommended for expanding or long-term cultures since the cells do not proliferate in regular culture.?

Recommended Medium
It is recommended to use Macrophage Medium (MaM, Cat. #1921) for culturing HHMa in vitro

Specifications:
Catalog No.
5340
Country of Manufacture
United States
Product Code
HHMa
Size/Quantity
1 x 10^6 cells in 1 ml volume
Product Use
HHMa are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping Info
Dry ice.
References
[1] Liaskou E, Wilson DV, Oo YH. (2012) "Innate immune cells in liver inflammation." Mediators Inflamm. 2012: 949157. [2] Bieghs V, Verheyen F, van Gorp PJ, Hendrikx T, Wouters K, Lutjohann D, Gijbels MJ, Febbraio M, Binder CJ, Hofker MH, Shiri-Sverdlov R. (2012) "Internalization of modified lipids by CD36 and SR-A leads to hepatic inflammation and lysosomal cholesterol storage in Kupffer cells." PLoS One. 7: e34378. [3] Tian Y, Jochum W, Georgiev P, Moritz W, Graf R, Clavien PA. (2006) "Kupffer cell-dependent TNF-alpha signaling mediates injury in the arterialized small-for-size liver transplantation in the mouse." Proc Natl Acad Sci U S A. 103: 4598-603. [4] Seki E, de Minicis S, Inokuchi S, Taura K, Miyai K, van Rooijen N, Schwabe RF, Brenner DA. (2009) "CCR2 promotes hepatic fibrosis in mice." Hepatology. 50: 185-97.