商品编号


ax0211



品牌


Axolbio



公司


Axolbio



公司分类


Huntington\'s




1 Ea



商品信息
1 vial of Neural Stem Cells (1.5 million cells/vial) and 1 x 30 mL Plating-XF Medium (ax0033). After thawing and plating, the neural stem cells terminally differentiate to acquire mature electrophysiological properties, and form functional synaptic network over a period of 40 ~ 50 days.
Description:



Model Huntington's Disease in a Dish







We have applied our innovative technology to develop human neural stem cells directly differentiated from?
Huntington’s Disease iPS cells
. ?Our cells allow Huntington's Disease researchers to build their own human cellular models in a disease-relevant genetic and tissue context.?

Our ready-to-use kits allow you to concentrate on your experiments rather than spending valuable time and resources generating Huntington's Disease neural
Progen
itors in-house. ?As part of the?
HD NSC kit
, you will receive?
Plating-XF Medium
?in addition to your vial of human neural stem cells to maximize cell vi
ABI
lity. ?

HD NSCs
?are ready made and easy to differentiate. ?They are capable of differentiating into a spectrum of cortical excitatory and inhibitory neurons and remain viable in culture for months, facilitating reproduc
IBL
e and long-term studies.

Whether you want to study Huntington's Disease pathogenesis at the cellular level, perform neurotoxicity testing or pre-clinical drug screening, we believe that?
HD NSCs
, alongside our optimized
?culture reagents
, provide the perfect culture system for your research success.
















Phase Contrast Image of Huntington's Disease Human Neural Stem Cells.?

The image was taken at Day 7
in vitro
. In the centre of the image you can see HD NSCs in a neural rosette formation. Neurite outgrowth can also be observed.?







Huntington's Disease Neural Stem Cells Express Neural Stem/
Progen
itor Cell
Marker
s.



HD NSCs were fixed after 7 days in culture and stained using our
immunocytochemisty protocol
. The neural stem cells were counterstained with
DAPI
to visualise the nuclei. The cells can be seen to express neural stem/
Progen
itor cell
Marker
s
Ki67
,
Nestin
and
Pax6
.






? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? DAPI
?
Pax6
?
Nestin



? ? ? ? ? ? ? ? ? ? ? ? ? DAPI
Nestin

Ki67










Advantages of Axol HD NSCs


Human cells derived from diagnosed Huntington's Disease patients



Available in industrial quantity



High purity neural stem cell population



Ready-made and easy to differentiate



Robust enough to undergo genetic manipulation



Capable of differentiating to a spectrum of cortical excitatory and inhibitory neurons



Remain viable in culture for months to facilitate reproduc
IBL
e and long term studies




Applications
?


Studying Huntington's Disease pathogenesis at the cellular level



Studying neural network formation and signal transmission in Huntington's Disease neural culture



Neurotoxicity testing



Preclinical drug efficacy testing



Co-culture studies with astrocytes












Product Specification:


Starting material

Fibroblasts



Donor gender

Female



Donor age at sampling

48 yrs



Donor age of onset

42 yrs



Reprogramming method

Retroviral



Induction method

Monolayer & chemically defined medium



Size

≥1.5 million cells



Kit components

1 vial of Neural Stem Cells (≥1.5 million cells) and 1 bottle of Neural Plating-XF Medium (30 mL)



Cell type

iPSC-derived neural stem cells



Growth properties

Adherent



Shipping conditions

Dry ice



Storage conditions

Liquid nitrogen