商品编号


0853



品牌


Sciencell



公司


Sciencell research lab



公司分类


Human Pluripotent Stem Cells




1 Ea

商品信息
Four qPCR controls are included in this kit to verify successful reverse transcription of messenger RNA (mRNA) to complementary DNA (
CDN
A), reveal the presence of genomic DNA (gDNA) contamination in
CDN
A samples, and detect qPCR inhibitor contamination.

The pluripotency of human embryonic and induced pluripotent stem cells (ESCs/iPSCs) are maintained by certain essential transcription factors
1
.
Sciencell
has created a convenient qPCR kit for the assessment of these key pluripotency factors to ensure the quality of your human ESCs/iPSCs. NANOG, SOX2 and POU5F1 qPCR primers included in the kit allow for the detection and quantification of ESCs/iPSCs pluripotency.

Note
:
all gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

Four qPCR controls are included in this kit to verify successful reverse transcription of messenger RNA (mRNA) to complementary DNA (
CDN
A), reveal the presence of genomic DNA (gDNA) contamination in
CDN
A samples, and detect qPCR inhibitor contamination. Good quality
CDN
A is a critical component for successful gene expression analysis.

Each primer set included in HSC-qPCR kit arrives lyophilized in a 2 mL vial. All primers are designed and tested under the same parameters: (i) an optimal annealing temperature of 65°C (with 2 mM Mg2+, and no DMSO); (ii) recognition of all known target gene transcript variants; and (iii) specific amplification of only one amplicon. Each primer set has been validated by qPCR by melt curve analysis and gel electrophoresis.

Specifications:
Catalog No.
0853
Country of Manufacture
United States
Product Code
HSC-qPCR
Size/Quantity
50 reactions
Product Use
HSC PCR kit is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Store in -20°C upon receipt. Avoid repeated freeze thaw cycles by making five aliquots at 180μL each.
Shipping Info
Dry ice.
References
[1] Lingyi Chen and George Q. Daley (2008) Molecular basis of pluripotency. Hum. Mol. Genet. 17 (R1): R23-R27.