商品编号


R5340



品牌


Sciencell



公司


Sciencell research lab



公司分类


Hematopoietic Cell System




1 Ea

商品信息
Isolated from postnatal day 2 rat liver. RHMa are cryopreserved after purification and delivered frozen. Each vial contains > 1 x 10^6 cells in 1 ml volume.

Macrophages are cells differentiated from circulating bone marrow-derived monocytes. Their main function is to remove cellular debris and inv
ADI
ng pathogens. Hepatic macrophages, which are also known as Kupffer cells, reside within the lumen of liver sinusoids. Hepatic macrophages protect the liver by responding to pathogens and metastatic cells, whilst tolerating harmless self and foreign antigens, which enter via blood flow through portal vein and hepatic artery [1]. Recent studies have shown that hepatic macrophages play an important role in fibrosis, liver inflammation, alcoholic liver disease, fatty liver disease, liver transplantation and more [2-5]. Macrophages isolated from fresh rat liver tissue serve as an excellent model for studying their functions under normal physiological and pathological conditions.

RHMa from ScienCell Research Laboratories are isolated from postnatal day 2 CD? IGS rat liver. RHMa are cryopreserved after purification and delivered frozen. Each vial contains > 1 x 10^6 cells in 1 ml volume. RHMa are characterized by immunofluorescence with antibody to F4/80. RHMa are negative for mycoplasma, bacteria, yeast, and fungi. RHMa is guaranteed to further culture in the condition provided by
Sciencell
Research Laboratories; however, RHMa are not recommended for expanding or long-term cultures since the cells do not proliferate in regular culture.

Recommended Medium

It is recommended to use Macrophage Medium (MaM, Cat. #1921) for culturing RHMa
in vitro
.

Specifications:
Catalog No.
R5340
Country of Manufacture
United States
Product Code
RHMa
Size/Quantity
1 x 10^6 cells/vial
Product Use
RHMa are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.
Shipping Info
Dry ice.
References
[1]. Wynn TA and Barron L. (2010). Semin Liver Dis. 30:245-57.[2]. Liaskou E, Wilson DV and Oo YH. (2012). Mediators Inflamm. 2012: 949157. [3]. Ajakaiye M, Jacob A, Wu R, Zhou M, et al. (2011). Biochem Biophys Res Commun. 409: 406-411.[4]. Bieghs V, Verheyen F, van Gorp PJ, Hendrikx T, et al. (2012) PLoS One. 7: e34378.[5]. Tian Y, Jochum W, Georgiev P, Moritz W, et al. (2006). Proc Natl Acad Sci U S A. 103: 4598-4603.[6]. Seki E, De Minicis S, Inokuchi S, Taura K, et al. (2009). Hepatology. 50: 185-197.[7]. Gordon S and Taylor P. (2005). Nature Reviews Immunology. 5:953-964.