商品编号


4827-30-K



品牌


Trevigen



公司


Trevigen, Inc



公司分类


Apoptosis



Size

30 Samples

商品信息

Description

The high cellularity of cardiac tissue presents problems in perme
ABI
lization, so the CardioTACS? Kit comes with two perme
ABI
lization reagents to provide options. The kit is based on DNA end-labeling using terminal deoxynucleotidyl transferase (TdT) and a modified nucleotide that is subsequently detected using our TACS Blue Label? detection system.
Trevigen
has developed an exclusive apoptosis grade TdT enzyme that when coupled with the TACS Blue Label? solution, provides labeling 20 to 50 times more sensitive than standard diaminobenzidine (DAB) labeling methods. To ensure ease of data interpretation when the numbers of apoptotic cells are low, CardioTACS? includes Nuclear Fast Red Counterstain which provides superb contrast to the TACS Blue Label? in apoptotic cells.

In addition, the kit includes TACS-Nuclease? to generate a positive control in your own sample. The positive control provides an internal control for perme
ABI
lization and labeling so additional optimization is nominal and the data can be interpreted with confidence. This complete kit provides all the reagents required for labeling including two perme
ABI
lization reagents, labeling and detection reagents, stop buffer, counterstain, TACS-Nuclease? reagent, and a protocol detailing incubation times and reagent concentrations.

?

FEATURES:


Performance tested on heart-derived samples.

Includes both Proteinase K and exclusive Cytonin? non-enzymatic perme
ABI
lization reagents.

Includes TACS-Nuclease? solution for preparing sample-dependent positive controls.

Helps resolve unique problems encountered when detecting apoptotic cardiac cells.


APPLICATIONS:


In situ
detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cardiac cells and tissues.

Assists in the identification of apoptotic morphologies.





Catalog # 4827-30-K includes :



Catalog Number

Description

Qty



4800-30-01

Proteinase K

1



4800-30-06

Streptavidin-HRP

1



4800-30-11

TACS-Blue Label?

1



4800-30-12

Blue Strep-HRP Diluent

1



4800-30-15

TACS-Nuclease?

1



4800-30-16

TACS-Nuclease? Buffer

1



4800-30-17

Nuclear Fast Red

1



4810-30-02

TACS? 2 TdT Labeling Buffer

1



4810-30-03

TACS? 2 TdT Stop Buffer

1



4810-30-04

TACS? 2 TdT dNTP

1



4810-30-05

TdT Enzyme

1



4810-30-14

50x Manganese Cation

1



4876-05-01

Cytonin?

1




?

The Influence of Age on Serum Concentrations of Cardiac Troponin I: Results in Rats, Monkeys, and Commercial Sera
Eugene H. Herman, Alan Knapton, Yongmin Liu, Steven E. Lipshultz, Joel Estis, John Todd, Ruth A. Woodward, Thomas Cochran, Jun Zhang, and Miriam C. Poirier
Toxicol Pathol, Jul 2014; 42: 888 – 896.
http://tpx.sagepub.com/cgi/content/abstract/42/5/888

“GSK-3β heterozygous knockout is cardioprotective in a knockin mouse
model of familial dilated cardiomyopathy”
“Rasha M. S. M. Mohamed, Sachio Morimoto, Islam A. A. E.-H. Ibrahim, Dong-Yun Zhan, Cheng-Kun Du,Masaki Arioka, Tatsuya Yoshihara, Fumi Takahashi-Yanaga, and Toshiyuki Sasaguri”
Am J Physiol Heart Circ Physiol, Jun 2016; 310: H1808 – H1815
http://ajpheart.physiology.org/cgi/content/abstract/310/11/H1808

“Phosphodiesterase 5 Inhibition Limits Doxorubicin-induced Heart Failure by Attenuating Protein Kinase G Iα Oxidation”
“Oleksandra Prysyazhna, Joseph Robert Burgoyne, Jenna Scotcher, Steven Grover, David Kass, and Philip Eaton”
J. Biol. Chem., Aug 2016; 291: 17427 – 17436.
http://www.jbc.org/cgi/content/abstract/291/33/17427