商品编号


AG-40T-0231-C050



品牌


Adipogen



公司


Adipogen



公司分类


Cancer



Size

50 ?g

商品信息


More Information



Product Details



Synonyms

Ubiquitin C-terminal Hydrolase CYLD; Deubiquitinating Enzyme CYLD; Ubiquitin Thioesterase CYLD; Ubiquitin-specific-processing Protease CYLD; CYLD1



Product Type

Protein



Properties



Source/Host

Sf21 cells



Sequence

Human CYLD Isoform 1 (Accession Nr. Q9NQC7-1) fused to a N-terminal His-tag.



Crossreactivity

Human



Formulation

Liquid. In HEPES, NaCl and DTT.



Other Product Data


Use:
Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 50-500nM.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Ubiquitin carboxyl-terminal hydrolase CYLD is a 956 amino acid (aa) member of the peptidase C67 protein family with a predicted molecular weight of 107 kDa. The mouse and rat CYLD orthologs share 95% and 94% aa sequence identity with the human protein, respectively. Two isoforms of CYLD have been identified, a full-length isoform and a second isoform that lacks aa 305-307 due to alternative splicing. Expression of CYLD has been reported in fetal brain as well as adult brain, heart, leukocytes, skeletal muscle, spleen and testis. CYLD acts as a deubiquitinase and removes K63-linked ubiquitin chains from multiple substrates including IκB, c-Jun, and c-Fos, resulting in the inhibition of NF-κB and JNK signaling. In some contexts, CYLD enhances mitosis entry and it has also been shown to delay G1/S phase entry suggesting that CYLD regulates multiple phases of the cell cycle. CYLD is recognized as a tumor suppressor and mutations in CYLD result in skin appendage syndromes including Brooke-Spiegle Syndrome, familial cylindromatosis and familial trichoepitheliomas type 1.