商品编号


LSK1200-25



品牌


Lifesci



公司


Life Sciences Advanced Technologies, Inc.



公司分类


cDNA Kit and T7T Kit




25 reactions

商品信息

Product Description

First-strand
CDN
A Synthesis Kit 25 Reaction Kit.

CONTENTS:

This kit contains sufficient reagents to perform 25 first strand
CDN
A syntheses with up to 2 mg of purified poly A(+)mRNA or 10 mg of total cell RNA per reaction.

STORAGE:

12 months at -20°C
12 months at -70°C

DESCRIPTION:

This First-strand
CDN
A Synthesis kit has been developed to maximize the synthesis of full-length
CDN
A. This kit produces an optimal template for DNA amplification techniques. Tested against a 7.5 kb synthetic poly A(+)RNA and a 9.8 kb native viral mRNA, 32% and 42% of the
CDN
As synthesized were full-length, respectively. Also,
CDN
As to a poly A(+)RNA ladder consisted of approximately 70% full length product (Fig. 1). The use of full-length
CDN
A templates for polymerase chain reaction (PCR) (1) affords equal avail
ABI
lity of all sequences for priming and amplification regardless of location. This kit includes RNase inhibitor, DTT solution, 5X reaction buffer containing nucleotides, and AMV reverse transcriptase enzyme. Pd(T)12-18 and random hexamer primers are also provided, together with RNase-free water and a polyadenylated RNA ladder as control template.

CDN
A SYNTHESIS


Figure 1. [32 P]-labeled
CDN
A to three RNA templates:

Lanes 1 and 2: 7.5 kb synthetic poly A(+)RNA primed with pd(T)12-18 and random hexamers, respectively.
Lanes 3 and 4: 9.8 kb tobacco vein mottling virus (TVMV) mRNA, primed with pd(T)12-18 and random hexamers, respectively.
Lanes 5 and 6: poly A(+)RNA ladder* primed with pd(T)12-18 and random hexamers, respectively.

*This polyA(+) RNA ladder is different from the one supplied in this kit.

FOR LABORATORY USE ONLY

Download details and protocol.