商品编号


P7520L



品牌


Enzymatics



公司


Enzymatics,inc



公司分类


Ultrapure Polymerases



Size

500 U

商品信息

VeraSeq? ULtra DNA Polymerase


Product Description

VeraSeq ULtra DNA polymerase is an engineered, ultra-
Thermo
stable, uracil-literate polymerase designed to maximize the speed, accuracy, and length of DNA synthesis during sequencing template preparation. The result is a novel enzyme that can read through uracil, extend a kilobase of sequence in 15 seconds and has an accuracy 25 times higher than Taq DNA Polymerase.

Source of Protein
A recombinant?
E. coli
?strain carrying the engineered VeraSeq ULtra gene.

Supplied in
20 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
St
ABI
lizer
50% glycerol
pH 7.4 @ 25°C

Supplied With:
5X VeraSeq Buffer II (B7102)
5X VeraSeq GC Buffer (B7130)

Unit Definition
One unit is defined as the amount of enzyme required to incorporate 10 nmoles of dNTPs into acid-insoluble form at 74°C in 30 minutes.



Quality Control Analysis

Unit Characterization Assay
Specific activity was measured using a 2-fold serial dilution method. Dilutions of enzyme were made in 1X reaction buffer and added to 50 ?L reactions containing activated calf thymus DNA; 25 mM TAPS (tris-[hydroxymethyl]-methyl-amino-propanesulfonic acid, sodium salt),pH 9.3 at 25°C; 50 mM KCl; 2 mM MgCl
2
; 1 mM β-mercaptoethanol; 200 ?M each dATP, dGTP, dTTP; and 100 ?M [
3
H]-dCTP (0.075 Ci/mmole). Reaction vessels were mixed and incubated at 74°C for 10 minutes.

Protein Concentration (OD
280
) Measurement
The enzyme was analyzed at OD
280
?using a
Nanodrop
spectrophotometer standardized with a 2.0 mg/ml BSA sample and blanked with product storage solution. The observed average measurement of 3 replicate samples was converted to mg/mL using an extinction coefficient of 134,130 and molecular weight of 97,754 Daltons.

SDS-Page (Physical Purity Assessment)
A concentrated sample of enzyme was loaded on a denaturing 4-20% Tris-Glycine SDS-PAGE gel flanked by a broad-range MW
Marker
and a 1:100 dilution of the sample. Following electrophoresis, the gel was stained and the samples compared to determine physical purity. The acceptance criteria for this test requires that the aggregate mass of contaminant bands in the concentrated sample do not exceed the mass of the protein of interest band in the dilute sample. Comparison between the concentrated and the diluted samples is used to evaluate percent purity.



Contamination Tests

Double-Stranded Endonuclease Activity
A reaction containing plasmid DNA incubated with enzyme solution for 4 hours at 37°C resulted in no visually discern
IBL
e conversion to nicked circular DNA as determined by agarose gel electrophoresis.

E.coli
16S rDNA Contamination Test
Replicate samples of enzyme were denatured and screened in a TaqMan qPCR assay for the presence of contaminating
E.coli
genomic DNA using oligonucleotide primers corresponding to the 16S rRNA locus. The acceptance criterion for the test is the threshold cycle count (C
t
) produced by the average of 3 replicate no template control samples. Based on the correlation between the no template control C
t
values, and standard curve data, the detection limit of this assay is <10 copies genome/sample.


View PDF Poster Instructions FAQ


Product Information



VeraSeq? ULtra DNA Polymerase


Part Number
P7520L
Price
$363
Concentration
2,000 U/ml
Unit Size
500 U


SDS

Available on request








Product Specification*


Storage Temperature
-25 to -15°C


Test

Specification


Purity (SDS-PAGE)
> 95%
Specific Activity
100,000 U/mg
DS Endonuclease
120 U = no conversion
E.coli
DNA Contamination
150 U < 10 copies





* For a detailed summary of assay conditions and data, refer to the Quality Controls Analysis section.

Additional Downloads

Product Note

Legal Disclaimer

This product is licensed from
Bio-Rad
Laboratories, Inc., under U.S. Pat. Nos. 6,627,424, 7,541,170, 7,560,260 and corresponding patents in other countries for use solely in DNA sequencing, DNA micro-array, and conventional PCR applications, including pre-amplification steps that are required for such applications, in the life science research fields but not real-time PCR or digital PCR.
In-vitro
diagnostics rights are available, please contact
Enzymatics
for more information.



Limitations of Use
This product was developed, manufactured, and sold for in vitro use only. The product is not suitable for admi
NIST
ration to humans or animals. SDS sheets relevant to this product are available upon request.