商品编号


BSAI-200



品牌


MCLab



公司


MCLAB.Inc



公司分类


Restriction Endonucleases



Concentration

100 U/ul


Size

5,000 units

商品信息



Recognition site:
GGTCTC(N)
1
Λ
CCAGAG(N)
5
Source:
An?
E. coli
?strain that carries the BsaI gene from ?
Bacillus stearo
Thermo
philus



Unit Definition
One unit is defined as the amount of enzyme required to digest 1 ?g of pXba DNA in 1 hour at 37°C in a total reaction volume of 50??l.



Reaction Condition
1X BsaI Buffer at 37°C

10x BsaI Buffer:
100 mM Tris-HCl (pH 8.5 at 37°C)
100 mM MgCl
2
1 M KCl
1 mg/ml BSA
Storage Buffer:

10 mM Tris-HCl
300 mM NaCl
1 mM DTT
0.1 mM EDTA
500 μg/ml BSA
50% Glycerol
pH 7.4 @ 25°C
Heat Inactivation

65°C for 20 min


Typical ligation/recut assay result:
More than 95% of the DNA fragments can be ligated after a 10-fold over-digestion of pXba DNA with BsaI, and more than 95% of these DNA fragments can be recut with BsaI.


Recommended storage condition:
?-20°C


Comments:
Blocked by overlapping
?dcm
?methylation. Cleavage of mammalian genomic DNA is blocked by some combinations of overlapping CpG methylation. Activity at 50°C is 100%.